Immediate early proteins of herpes simplex virus transiently repress viral transcription before subsequent activation.

ABSTRACT: This study was undertaken to further clarify the roles of HSV-1 immediately early (IE) genes ICP4, ICP0, and ICP22 in early viral transcription. Precision nuclear Run On followed by deep Sequencing (PRO-Seq) was used to identify sites of Pol activity to high resolution on the genomes of HSV-1 viruses bearing mutations in a0, a4 or a22/US1, and corresponding viruses in which these loci were genetically restored. The results indicated that prior to initiating activation of transcription, IE genes first mediate transcriptional repression.

ORGANISM(S): Homo sapiens

PROVIDER: GSE202363 | GEO | 2022/10/18

REPOSITORIES: GEO