Genomics

Dataset Information

0

H3K9K14ac ChIP-chip in lung cancer cells treated with histone deacetylase inhibitor


ABSTRACT: Lung cancer is the leading cause of cancer mortality worldwide, yet the therapeutic strategy for advanced non-small cell lung cancer (NSCLC) is limitedly effective. In addition, validated histone deacetylase (HDAC) inhibitors for the treatment of solid tumors remain to be developed. Here, we propose a novel HDAC inhibitor, OSU-HDAC-44, as a chemotherapeutic drug for NSCLC. OSU-HDAC-44 was a pan-HDAC inhibitor and exhibits 3-4 times more effectiveness than suberoylanilide hydroxamic acid (SAHA) in suppressing cell viability in various NSCLC cell lines. Upon OSU-HDAC-44 treatment, mitosis and cytokinesis were inhibited and subsequently led to mitochondria-mediated apoptosis. The cytokinesis inhibition resulted from OSU-HDAC-44-mediated degradation of mitosis and cytokinesis regulators Auroroa B and survivin. The deregulation of F-actin dynamics induced by OSU-HDAC-44 was associated with reduction in RhoA activity resulting from srGAP1 induction. Chromatin-immunoprecipitation-on-chip analysis revealed that OSU-HDAC-44 induced chromatin loosening and facilitated transcription of genes involved in crucial signaling pathways such as apoptosis, axon guidance and protein ubiquitination. Finally, OSU-HDAC-44 efficiently inhibited A549 xenograft tumor growth and induced acetylation of histone and non-histone proteins and apoptosis in vivo. Collectively, our data provide compelling evidence that OSU-HDAC-44 is a potent HDAC targeted inhibitor and can be tested for NSCLC chemotherapy.

ORGANISM(S): Homo sapiens

PROVIDER: GSE20304 | GEO | 2010/12/22

SECONDARY ACCESSION(S): PRJNA125343

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2010-12-22 | E-GEOD-20304 | biostudies-arrayexpress
2008-04-05 | E-GEOD-9987 | biostudies-arrayexpress
2008-03-20 | GSE9974 | GEO
2008-04-05 | E-GEOD-9974 | biostudies-arrayexpress
| 2081555 | ecrin-mdr-crc
| 5791 | ecrin-mdr-crc
2008-03-20 | GSE9976 | GEO
2022-05-04 | MTBLS682 | MetaboLights
2008-06-11 | E-GEOD-10089 | biostudies-arrayexpress
2008-01-08 | GSE10089 | GEO