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PPARd target genes in MEFs

ABSTRACT: The PPAR (Peroxisome proliferator-activated receptor) family of nuclear receptors has three members: PPARg, PPARa and PPARd. Although they share similar structures, their biological functions are distinct. PPARg controls lipid storage and adipogenesis, while PPARd is associated with fat burning. The highly specific synthetic ligand for PPARd, GW501516, is a promising drug candidate for obesity and diabetes. Here we use Affymetrix microarray to analyze gene expression profile in mouse embryo fibroblasts treated with 100 nM GW501516 for 0, 2, 8 and 24 hours. These data may provide new clues into the molecular mechanism by which GW501516 ameliorates obesity and diabetes.

ORGANISM(S): Mus musculus

PROVIDER: GSE20371 | GEO | 2013/12/31

SECONDARY ACCESSION(S): PRJNA125473

REPOSITORIES: GEO

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PPARd target genes in MEFs

Project description:The PPAR (Peroxisome proliferator-activated receptor) family of nuclear receptors has three members: PPARg, PPARa and PPARd. Although they share similar structures, their biological functions are distinct. PPARg controls lipid storage and adipogenesis, while PPARd is associated with fat burning. The highly specific synthetic ligand for PPARd, GW501516, is a promising drug candidate for obesity and diabetes. Here we use Affymetrix microarray to analyze gene expression profile in mouse embryo fibroblasts treated with 100 nM GW501516 for 0, 2, 8 and 24 hours. These data may provide new clues into the molecular mechanism by which GW501516 ameliorates obesity and diabetes. Wild type mouse embryonic fibroblasts (MEFs) stably infected with retroviruses MSCVpuro expressing PPARd were plated at 0.5 million per 10 cm dish. After overnight incubation, cells were treated with 100nM GW501516 for 0h, 2h, 8h and 48h. Cells were collected at subconfluent condition. Total RNAs were sequentially purified with Trizol (Invitrogen) and RNeasy kit (Qiagen) and analyzed in triplicate on Mouse Genome 430 2.0 Array (Affymetrix) at NIDDK Microarray Core Facility following standard protocols.

2013-12-31 | E-GEOD-20371 | biostudies-arrayexpress

Gene expression profile of peroxisome proliferator-activated receptor delta (Ppard)-overexpressing RAW 264.7 macrophages treated vehicle, GW501516 or interleukin-4 (Il-4)

Project description:Investigation of whole genome gene expression level changes in GW501516 (a Ppard ligand) or Il-4 treated Ppard-overexpressing RAW 264.7 macrophages as compared to vehicle. Alternative activation of adipose tissue resident macrophages inhibits obesity-induced metabolic inflammation. In the current study we profile genes regulated by two M2 inducers, Il-4 or Ppard agonists and find that alternative activation promotes the cell survival program, while inhibiting that of inflammation-related cell death.

2017-06-21 | GSE100237 | GEO

Expression data from MMTV-PPARd mice, 1 week GW501516 treatment

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Expression data from MMTV-PPARd mice, 1 week GW501516 treatment

Project description:The role of murine peroxisome proliferator-activated receptor-delta (PPARd) in mammary tumorigenesis was assessed. Microarrays were used to analyse global gene expression to determine changes in MMTV-PPARd transgenic mice versus wild-type mice and the effect of GW501516. RNA was isolated (RNeasy Mini Kit, Qiagen) from mammary gland tissue of transgenic and wild-type mice maintained on normal rodent chow or 0.005% GW501516

2013-05-31 | E-GEOD-34109 | biostudies-arrayexpress

PPARD in MDM - chipseq

Project description:PPARD in MDM - chipseq

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Expression profiling, MDA-MB-231-luc2 response to GW501516

Project description:MDA-MB-231-luc2 cells were treated with the PPARd specific agonist GW501516 and the resulting expression changes profiled.

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PPARD is a critical inhibitor of cartilage growth in external ears [RNA-Seq]

Project description:In this study, we uncover the molecular mechanism that PPARD transactive-deficient pigs have large-eared feature. Evidently from this work, PPARD plays a vital role on inhibiting cartilage growth by regulating the transcription of critical genes for chondrogenesis in ligand dependent manner; the activation of sPPARD speeds up apoptosis in CSPCs, terminal differentiation of chondroblasts and matrix degradation in auricular cartilage. The transactive-deficient PPARD has not the capability to slow down cartilage growth and consequently causes that ear size of carried animals increases at a high growth rate. As the role of PPARD in cartilage is similar in pigs, mice and humans, and since cartilage development is well-conserved in animals, our data helps to better understanding of cartilage development and treatment of cartilage-related diseases.

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PPARD is a critical inhibitor of cartilage growth in external ears [ChIP-Seq]

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The roles of ppar-delta and ppar-gamma in regulating the metabolic switch between oxidation and storage of fats in white adipose tissue

Project description:Obesity is a major risk factor for the development of insulin resistance and type II diabetes. The nuclear receptors PPAR delta and PPAR gamma play a central role in regulating metabolism in adipose tissue, as well as being targets for the treatment of insulin resistance. The metabolic effects of PPAR delta and PPAR gamma activation have been examined both in vivo in white adipose tissue from ob/ob mice and in vitro in cultured 3T3-L1 adipocytes using a combined 1H NMR spectroscopy and mass spectrometry metabolomic methodology to understand the contrasting roles of these receptors. These steady state measurements were supplemented with 13C-stable isotope substrate labeling to assess fluxes, respirometry and transcriptomic microarray analysis. The metabolic effects of the two receptors were readily distinguished, with PPAR gamma ?activation characterised by increased fat storage and fat synthesis/elongation, while activation of PPAR delta caused increased fatty acid beta-oxidation, TCA cycle rate and oxidation of extracellular branch chain amino acids. Stimulated glycolysis and increased desaturation of fatty acids were the only common pathways. PPAR delta has a role as an anti-obesity target as well as an anti-diabetic. Total RNA obtained from cultured 3T3-L1 cells treated for 48 hours with either DMSO control, GW610742 PPARd agonist or GW347845 PPARg agonist and compared.

2011-07-13 | E-GEOD-26207 | biostudies-arrayexpress

Mus musculus

Project description:Expression data from MMTV-PPARd mice, 1 week GW501516 treatment

| PRJNA149889 | ENA