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Modeling human extraembryonic mesoderm cells using naive pluripotent stem cells [scRNA_seq_timecourse_naiveTSC]

ABSTRACT: time course scRNAseq of naïve to trophoblast stem cells and extraembryonic mesoderm conversion and D70 sorted extraembryonic mesoderm. Samples were collected at day 0, 1, 2, 4, 8, 13 and 18.

ORGANISM(S): Homo sapiens

PROVIDER: GSE204779 | GEO | 2022/08/01

REPOSITORIES: GEO

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Modeling human extraembryonic mesoderm cells using naive pluripotent stem cells [scRNA-seq]

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Modeling human extraembryonic mesoderm cells using naive pluripotent stem cells [scATAC-seq]

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RNA-seq of H9 cell line during differentiation from naïve extraembryonic endoderm to extraembryonic mesoderm.

Project description:Extraembryonic mesoderm (ExM) is one of the first cell types that emerges during embryogenesis and constitutes essential supportive tissues for the pregnancy. Primate ExM is known to form prior to gastrulation, unlike its murine counterpart which is derived from the primitive streak. Based on the embryonic morphology and the proximity of ExM to the extraembryonic endoderm (hypoblast), we hypothesised that ExM can be derived in vitro from the naïve extraembryonic endoderm (nEnd) cell line. We applied a mesoderm differentiation protocol, which has been reported to induce ExM from mouse epiblast stem cells, on human nEnd and analysed the transcriptome on day 0, 1, 2, 8 and 15.

2023-06-29 | E-MTAB-12517 | biostudies-arrayexpress

scRNA-seq of mouse EpiSCs (epiblast stem cells) differentiated to extraembryonic mesoderm in vitro, in a monolayer

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CDX2 dose-dependently influences the GRN underlying vascularization in human extraembryonic mesoderm-like cells

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Modeling human extraembryonic mesoderm cells using naive pluripotent stem cells

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-08-01 | GSE191286 | GEO

Conversion of murine extraembryonic trophoblast stem cells into induced pluripotent stem cells

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