Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression profile at single cell level of fibroblasts from untreated (UT) and imiquimod (IMQ)-induced mouse skin.

ABSTRACT: We used single-cell RNA sequencing (scRNA-seq) to analyze the heterogeneity of dermal fibroblasts in homeostasis and skin inflammation.

ORGANISM(S): Mus musculus

PROVIDER: GSE205020 | GEO | 2022/06/01

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Gene expression profile at single cell level of fibroblasts from untreated (UT) and imiquimod (IMQ)-induced mouse skin.

Project description:Gene expression profile at single cell level of fibroblasts from untreated (UT) and imiquimod (IMQ)-induced mouse skin.

| PRJNA843084 | ENA

Gene expression profile at single cell level of newborn and adult mouse dorsal skin organoids

Project description:The formation of tissue patterns is critical for organ functions in development and regeneration. To advance the use of organoids for organ regeneration, we learn principles that govern the skin organoids to regenerate hairs. We show the formation of epidermal-dermal coupled cysts function as competent morphogenetic units (CMU) which harbor the ability of skin organoids to regenerate. ScRNA-sequencing shows the emergence of cell types and new cell interactions during CMU formation. In newborn skin organoids, epidermal cells undergo apical-basal polarization via the IFNr-PKR-PKC signaling module. Dermal-Tgfb regulates Gsk3 to establish basement membranes between the epidermal cyst and dermal cells. Meanwhile, VEGF signaling mediates dermal cell attachment to the cyst. Adult cells cannot form organoids but can be induced to generate CMUs and regenerate hairs by adding IFNr or VEGF. We compare the similar principles and different paths used to establish morphogenetic competency in developing skin, wound-induced hair neogenesis, and organoids.

2023-09-13 | GSE215980 | GEO

Gene expression profile at single cell level of primary keloid fibroblasts treated with various radiotherapy modalities

Project description:Keloids are benign dermal tumors that arise from abnormal wound healing processes following skin lesions. Postoperative radiotherapy (PORT) is a clinically effective measure to reduce recurrence rates of keloid. We used single cell RNA sequencing (scRNA-seg) to analyze the different of primary keloid fibroblasts treated with various radiation modalities.

2024-04-07 | GSE261116 | GEO

RNA-seq of untreated and IMQ treated mouse back skin in control and Ovol1 deficient mice

Project description:Skin constitutes the outer permeability barrier that protects the body from dehydration and a myriad of external assaults. Epidermal keratinocytes act as the first line of innate immune defense, and barrier defects underlie common inflammatory skin diseases. However, the molecular mechanisms that maintain barrier integrity when skin is under challenge to regulate the interplay between epidermal and immune cells are not fully understood. Here we report upregulated expression of transcriptional repressorencoding Ovol1 in epidermal cells of inflamed skin, and its functional importance in maintaining barrier integrity of physically or chemically challenged skin. Following stimulation with imiquimod, Ovol1-deficient mice exhibit significantly aggravated epidermal hyperplasia and psoriasis-like skin inflammation featuring persistent neutrophil accumulation. Using bulk and single-cell RNA-sequencing, we characterize molecular changes in epidermal, fibroblasts, and immune cells that reflect altered epidermal proliferation and differentiation and/or significantly enhanced inflammatory responses as consequences of Ovol1 deletion. We identify both proliferation/differentiation-regulating and neutrophil-attracting chemokine genes as candidate direct targets of Ovol1. Finally, we provide evidence for altered IL-1a signaling in the microenvironment of Ovol1- deficient inflamed skin that functionally contributes to neutrophil accumulation and epidermal hyperplasia. Collectively, our study demonstrates a protective role for an epidermally expressed, disease-linked transcription factor in coordinating robust barrier maintenance with suppression of skin inflammation.

2021-10-27 | GSE158112 | GEO

Transcriptomic comparison of fibroblasts derived from healthy and psoriatic human skin

Project description:Dermal fibroblasts were isolated from healthy human skin or chronic psoriatic plaques for cultivation, which were subsequently subjected to RNA-Seq.

2022-10-18 | E-MTAB-11098 | biostudies-arrayexpress

Gene expression profiles from cultured skin fibroblasts of two MAF mutation-positive subjects

Project description:Total RNA was obtained from cultured skin fibroblasts of two MAF mutation-positive subjects and control (dermal adult skin fibroblasts, ATCC PCS-201-012) (each in duplicates), and was subjected for gene expression profiling to identify differentially expressed genes (DEG).

2015-07-31 | E-MTAB-3250 | biostudies-arrayexpress

RNA-seq analysis of IMQ-treated wt and Trex2-/- skin

Project description:TREX2 is a keratinocyte specific 3â-deoxyribonuclease that participates in the maintenance of skin homeostasis upon damage. This transcriptome analysis identified multiple genes and pathways deregulated by TREX2 loss in the IMQ-induced psoriasis-like model in mouse skin. mRNA sequencing of 5 biological replicates of skin from wild-type mice treated with Imiquimod and 6 of Trex2 knockout mice treated with Imiquimod

2016-09-07 | E-GEOD-74528 | biostudies-arrayexpress

Gene expression analysis between primary keratinocytes and primary dermal fibroblasts obtained from the same skin sample

Project description:Gene expression profile was obtained from primary keratinocytes and primary dermal fibroblasts obtained from the same skin sample

2016-09-01 | GSE85803 | GEO

Stump project -- mRNA fibroblasts; Volar versus non-volar acral skin gene expression

Project description:We were interested in defining the gene signature of volar skin. Dermal fibroblasts were expanded from punch biopsies of skin. We collected RNA from fibroblasts of palms, backs of hand, soles and backs of feet and submitted for Affymetrix Exon arrays. 3 replicates of each site from distinct human donors were included; because of 1 failed sample(Palm_rep2), total of 11 samples analyzed

2013-07-03 | E-GEOD-39266 | biostudies-arrayexpress

Microarray on cell populations sorted from the imiquimod-treated skin

Project description:The imiquimod model is used to induce the skin production of IL-22 and to characterize the cells that produce IL-22 in the skin after inflammatory stimulus. This experiment is performed on skin of C57Bl/6 mice treated with imiquimod to induce psoriasis-like lesions. After 5 days of imiquimod treatment, we analysed the heamatopoietic cells that infiltrate the skin and we compared the gene signature of three T cell populations: CD4+ T cells, CD4-CD8-(DN) T cells and gd T cells

2016-08-09 | E-MTAB-4453 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data