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Genome-wide assessment of DNA methylation alterations induced by superovulation, sexual immaturity and in vitro follicle growth in mouse blastocysts

ABSTRACT: In their attempt to fulfill the wish of having children, women who suffer from fertility issues often undergo assisted reproductive technologies (ART) such as ovarian stimulation, which has been associated with adverse health outcomes and imprinting disorders in children. However, given the crucial role of exogenous hormone stimulation in improving human infertility treatments, a more comprehensive analysis of the potential impacts on DNA methylation in embryos following ovarian stimulation is needed. Here, we provide genome-wide DNA methylation profiles of blastocysts generated after superovulation of prepubertal or adult mice, compared with blastocysts derived from non-stimulated adult mice. Additionally, we assessed the impact of the in vitro growth and maturation of oocytes on methylation in blastocysts.

ORGANISM(S): Mus musculus

PROVIDER: GSE205097 | GEO | 2023/01/19

REPOSITORIES: GEO

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Genome-wide DNA methylation assessment in mouse oocytes: effects of in vitro growth, superovulation and sexual immaturity

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2019-12-23 | GSE128656 | GEO

The use of a brief synchronization treatment after weaning, combined with superovulation, has moderate effects on the gene expression of surviving pig blastocysts

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Genome-wide DNA methylation assessment in mouse oocytes: effects of in vitro growth, superovulation and sexual immaturity

Project description:Genome-wide DNA methylation assessment in mouse oocytes: effects of in vitro growth, superovulation and sexual immaturity

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Analysis of gene expression during mouse preimplantation development in vitro, with or without a background of exogenous ovarian stimulation (superovulation)

Project description:Among the different procedures of the assisted reproductive technologies (ARTs), the purpose of ovarian stimulation with gonadotropins is to maximize the number of oocytes retrieved, and facilitate scheduling and planning of patient treatment. However, the doses of the gonadotropins required to increase the number of growing ovarian follicles, and, thereby, the number of oocytes, have been found to affect the quality of embryos in the mouse model of human reproduction (PMID 16569317, PMID 22802074, PMID 11387298, PMID 11157810). In order to clarify this issue, we compared the gene expression of mouse embryos with vs without a background of ovarian stimulation with PMSG and hCG. Pronuclear oocytes were cultured in KSOM(aa) and developmental stages were collected based on morphology. Analysis of transcriptome signal intensities showed similar profiles and produced the expected clustering (one cluster from oocyte to 2-cell stage, another cluster from 4-cell stage to blastocyst) regardless of whether the embryos were produced in stimulated or non-stimulated ovarian cycles; however, the expression levels of some genes were different depending on the ovarian stimulation.

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