Transcriptional response of Candida dubliniensis during hypha formation and environmental change
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ABSTRACT:
ORGANISM(S): Candida dubliniensis
PROVIDER: GSE20537 | GEO | 2010/02/27
SECONDARY ACCESSION(S): PRJNA125157
REPOSITORIES: GEO
Similar Datasets
Project description:Transcriptional response of Candida dubliniensis during hypha formation and environmental change (temperature, pH, density and nutrients). Transcript profiling of C. dubliniensis identified a core shared transcriptional response with C. albicans during hypha formation and growth at alkaline pH. However, C. albicans expresses several unique hypha-specific genes, including ALS3, HYR1 and SAP4 and 5. Transcript profiling also revealed a novel role for NRG1 in regulating ferric reductases in C. dubliniensis. 8 experimental parameters were analysed, each carried out in quadruplicate. Dye-swaps were performed.
2010-02-26 | E-GEOD-20537 | biostudies-arrayexpress
Project description:Candida albicans and Candida dubliniensis are closely related species displaying differences in virulence and genome content, therefore providing potential opportunities to identify novel C. albicans virulence genes. C. albicans gene arrays were used for comparative analysis of global gene expression in the two species in reconstituted human oral epithelium (RHE). C. albicans (SC5314) showed upregulation of hypha-specific and virulence genes within 30 min postinoculation, coinciding with rapid induction of filamentation and increased RHE damage. C. dubliniensis (CD36) showed no detectable upregulation of hypha-specific genes, grew as yeast, and caused limited RHE damage. Several genes absent or highly divergent in C. dubliniensis were upregulated in C. albicans. One such gene, SFL2 (orf19.3969), encoding a putative heat shock factor, was deleted in C. albicans. ΔΔsfl2 cells failed to filament under a range of hypha-inducing conditions and exhibited greatly reduced RHE damage, reversed by reintroduction of SFL2 into the ΔΔsfl2 strain. Moreover, SFL2 overexpression in C. albicans triggered hyphal morphogenesis. Although SFL2 deletion had no apparent effect on host survival in the murine model of systemic infection, ΔΔsfl2 strain-infected kidney tissues contained only yeast cells. These results suggest a role for SFL2 in morphogenesis and an indirect role in C. albicans pathogenesis in epithelial tissues.
2008-11-14 | GSE13318 | GEO
Project description:Candida albicans and Candida dubliniensis are closely related species displaying differences in virulence and genome content, therefore providing potential opportunities to identify novel C. albicans virulence genes. C. albicans gene arrays were used for comparative analysis of global gene expression in the two species in reconstituted human oral epithelium (RHE). C. albicans (SC5314) showed upregulation of hypha-specific and virulence genes within 30 min postinoculation, coinciding with rapid induction of filamentation and increased RHE damage. C. dubliniensis (CD36) showed no detectable upregulation of hypha-specific genes, grew as yeast, and caused limited RHE damage. Several genes absent or highly divergent in C. dubliniensis were upregulated in C. albicans. One such gene, SFL2 (orf19.3969), encoding a putative heat shock factor, was deleted in C. albicans. ΔΔsfl2 cells failed to filament under a range of hypha-inducing conditions and exhibited greatly reduced RHE damage, reversed by reintroduction of SFL2 into the ΔΔsfl2 strain. Moreover, SFL2 overexpression in C. albicans triggered hyphal morphogenesis. Although SFL2 deletion had no apparent effect on host survival in the murine model of systemic infection, ΔΔsfl2 strain-infected kidney tissues contained only yeast cells. These results suggest a role for SFL2 in morphogenesis and an indirect role in C. albicans pathogenesis in epithelial tissues.
2008-11-14 | GSE13345 | GEO
Project description:Candida albicans and Candida dubliniensis are closely related species displaying differences in virulence and genome content, therefore providing potential opportunities to identify novel C. albicans virulence genes. C. albicans gene arrays were used for comparative analysis of global gene expression in the two species in reconstituted human oral epithelium (RHE). C. albicans (SC5314) showed upregulation of hypha-specific and virulence genes within 30 min postinoculation, coinciding with rapid induction of filamentation and increased RHE damage. C. dubliniensis (CD36) showed no detectable upregulation of hypha-specific genes, grew as yeast, and caused limited RHE damage. Several genes absent or highly divergent in C. dubliniensis were upregulated in C. albicans. One such gene, SFL2 (orf19.3969), encoding a putative heat shock factor, was deleted in C. albicans. ÎÎsfl2 cells failed to filament under a range of hypha-inducing conditions and exhibited greatly reduced RHE damage, reversed by reintroduction of SFL2 into the ÎÎsfl2 strain. Moreover, SFL2 overexpression in C. albicans triggered hyphal morphogenesis. Although SFL2 deletion had no apparent effect on host survival in the murine model of systemic infection, ÎÎsfl2 strain-infected kidney tissues contained only yeast cells. These results suggest a role for SFL2 in morphogenesis and an indirect role in C. albicans pathogenesis in epithelial tissues. Global gene expression in Candida albicans SC5314 or Candida dubliniensis CD36 on reconstituted human oral epithelium (RHE) 90 min postinoculation. Gene expression on RHE was compared to gene expression on polycarbonate filter (PCF; used as RHE support matrix) 90 min postinoculation. Performed in two biological replicates with reciprocal dye swaps for each biological replicate. Gene expression of Candida cells on RHE was normalized to gene expression in reference control (PCF); log2 ratios were calculated by dividing spot intensity of experimental by that of the reference control.
2010-05-16 | E-GEOD-13345 | biostudies-arrayexpress
Project description:Candida albicans and Candida dubliniensis are closely related species displaying differences in virulence and genome content, therefore providing potential opportunities to identify novel C. albicans virulence genes. C. albicans gene arrays were used for comparative analysis of global gene expression in the two species in reconstituted human oral epithelium (RHE). C. albicans (SC5314) showed upregulation of hypha-specific and virulence genes within 30 min postinoculation, coinciding with rapid induction of filamentation and increased RHE damage. C. dubliniensis (CD36) showed no detectable upregulation of hypha-specific genes, grew as yeast, and caused limited RHE damage. Several genes absent or highly divergent in C. dubliniensis were upregulated in C. albicans. One such gene, SFL2 (orf19.3969), encoding a putative heat shock factor, was deleted in C. albicans. ÎÎsfl2 cells failed to filament under a range of hypha-inducing conditions and exhibited greatly reduced RHE damage, reversed by reintroduction of SFL2 into the ÎÎsfl2 strain. Moreover, SFL2 overexpression in C. albicans triggered hyphal morphogenesis. Although SFL2 deletion had no apparent effect on host survival in the murine model of systemic infection, ÎÎsfl2 strain-infected kidney tissues contained only yeast cells. These results suggest a role for SFL2 in morphogenesis and an indirect role in C. albicans pathogenesis in epithelial tissues. Global gene expression in Candida albicans SC5314 or Candida dubliniensis CD36 on reconstituted human oral epithelium (RHE) 30 min postinoculation. Gene expression on RHE was compared to gene expression in cells used as inoculum (0 min). Performed in three biological replicates with dye swaps across the biological replicates. Gene expression of Candida cells on RHE was normalized to gene expression in reference control (0 min); log2 ratios were calculated by dividing spot intensity of experimental by that of the reference control.
2010-05-16 | E-GEOD-13318 | biostudies-arrayexpress
Project description:mRNA profiles of Candida albicans wild-type (CAF2-1),cys4 mutant and CYS4 integrated strains were generated by deep sequencing, in triplicate, using Illumina HiSeq X Ten. The sequence reads that passed quality filters were analyzed at the transcript isoform level. qRT–PCR validation was performed using SYBR Green assay. We find that filament or biofilm-specific regulators such as UME6, TEC1 and BRG1 and adhesins such as hypha-specific genes ECE1 and ALS3 were downregulated and negative hyphae regulator NRG1 was upregulated in cys4 mutant. Genes invovled in cell wall synthesis and oxidative stress response were also accordingly regulated in cys4 mutant.
2022-06-01 | GSE137530 | GEO
Project description:In order to investigate the biology of chlamydospores, we compared global gene expression in C. dubliniensis yeast cells and chlamydospores. This revealed that 11% of the genes greater than 2-fold upregulated in chlamydospores were genes regulated by the transcriptional repressor Nrg1. These mainly included genes involved in glucose metabolism and glucose transport. Other upregulated genes included genes involved in stress response, cell cycle control, chromatin regulation, chromosome rearrangements, DNA repair-recombination and pheromone response. Genes related to mitochondrial respiratory functions and biogenesis were found to be downregulated in chlamydospores. Our data confirm that chlamydospores represent a specialised growth form of C. albicans and C. dubliniensis.
2010-09-01 | GSE21173 | GEO
Project description:In order to investigate the biology of chlamydospores, we compared global gene expression in C. dubliniensis yeast cells and chlamydospores. This revealed that 11% of the genes greater than 2-fold upregulated in chlamydospores were genes regulated by the transcriptional repressor Nrg1. These mainly included genes involved in glucose metabolism and glucose transport. Other upregulated genes included genes involved in stress response, cell cycle control, chromatin regulation, chromosome rearrangements, DNA repair-recombination and pheromone response. Genes related to mitochondrial respiratory functions and biogenesis were found to be downregulated in chlamydospores. Our data confirm that chlamydospores represent a specialised growth form of C. albicans and C. dubliniensis. 2 experimental parameters were analysed (chlamydospores, yeast cells). Three biological replicates each, one technical replicate.
2010-09-01 | E-GEOD-21173 | biostudies-arrayexpress
Project description:The purpose of this experiment was to identify the genes bound by the regulator Ndt80 in a C. albicans x C. dubliniensis hybrid during biofilm formation. C. albicans or C. dubliniensis Ndt80 was tagged with a c-myc epitope in a C. albicans x C. dubliniensis hybrid and the immunoprecipitation of the tagged strain was compared to that of an untagged control hybrid strain.
2021-04-14 | GSE161134 | GEO
Project description:This SuperSeries is composed of the following subset Series: GSE13318: Expression profiling of Candida albicans and Candida dubliniensis in reconstituted human oral epithelium 30 min p.i. GSE13345: Expression profiling of Candida albicans and Candida dubliniensis in reconstituted human oral epithelium 90 min p.i. GSE13352: Comparative expression profiling in Candida albicans and Candida dubliniensis in inocula, RHE, and PCF cultures Refer to individual Series
2010-05-16 | E-GEOD-13593 | biostudies-arrayexpress