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TAL1 knock down in Jurkat cells

ABSTRACT: We used microarray profiling in Jurkat cells to uncover TAL1 dependent genes in a leukemic context.

ORGANISM(S): Homo sapiens

PROVIDER: GSE20544 | GEO | 2010/12/14

SECONDARY ACCESSION(S): PRJNA129857

REPOSITORIES: GEO

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TAL1 knock down in Jurkat cells

Project description:We used microarray profiling in Jurkat cells to uncover TAL1 dependent genes in a leukemic context. Clonal Jurkat lines expressing a Doxycycline (Dox) -inducible shRNA against TAL1 coding sequence were generated. A clone in which Dox treatment led to 80% decrease of TAL1 mRNA was chosen.

2010-12-14 | E-GEOD-20544 | biostudies-arrayexpress

TAL1 knock down in human erythroid progenitors

Project description:We used microarray profiling in erythroid cells to uncover TAL1 dependent genes in a hematopoietic differentiation context.

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TAL1 knock down in human erythroid progenitors

Project description:We used microarray profiling in erythroid cells to uncover TAL1 dependent genes in a hematopoietic differentiation context. Differentiated ex vivo hematopoietic multipotential progenitors isolated from adult peripheral blood. The knockdown of TAL1 (KD) was induced in pro-erythroblasts (Days 8 and 9 of differentiation) using lentivirus-delivered shRNA. A scramble (scr) shRNA sequence was used as a negative control.

2010-12-14 | E-GEOD-20545 | biostudies-arrayexpress

TAL1 knock down

Project description:This SuperSeries is composed of the following subset Series: GSE20544: TAL1 knock down in Jurkat cells GSE20545: TAL1 knock down in human erythroid progenitors Refer to individual Series

2010-12-14 | E-GEOD-20546 | biostudies-arrayexpress

4C-seq analysis in TAL1-FKBP12 Jurkat cells

Project description:4C analysis was performed in TAL1-FKBP12 Jurkat cells to analyze chromatin-chromatin interactions after DMSO or dTAG-13 treatment.

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H3K27ac Hi-ChIP analysis in TAL1-FKBP12 Jurkat cells

Project description:H3K27ac Hi-ChIP analysis was performed in TAL1-FKBP12 Jurkat cells to analyze active chromatin-chromatin interactions after DMSO or dTAG-13 treatment.

2023-11-20 | GSE225937 | GEO

ChIP-seq analysis for TAL1-FKBP12 Jurkat cells

Project description:ChIP-seq analysis was performed in a Jurkat cell line to analyze DNA bindings of TAL1-FKBP12 protein using an anti-HA antibody after DMSO or dTAG-13 treatment.

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Cut&Run analysis for transcription factors in TAL1-FKBP12 Jurkat cells

Project description:Cut&Run analysis was performed in TAL1-FKBP12 Jurkat cells to analyze DNA bindings of GATA3 and RUNX1 after DMSO or dTAG-13 treatment.

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shRNA mediated knock-down of Tal1 in human Endothelial Colony Forming Cells (ECFCs)

Project description:Endothelial colony-forming cells (ECFCs) have been reported as promising cells for regenerative medicine thanks to their angiorepair properties. Transcription factors are primary determinants of the functional capacity of the cells and TAL1 has been shown as a critical regulator of endothelial lineage in both development and adult life. However, only few (three) TAL1 targets have been identified so far in mouse and human endothelial cells. This microarray experiment, where TAL1 expression was knocked-down, was designed to identify TAL1-dependent genes in primary human endothelial stem/progenitor cells.

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Project description:miRNA profiling of Jurkat T-ALL cells after knockdown with two control shRNAs and two shRNAs targeting TAL1 to identify miRNAs that are regulated by TAL1

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