A truncated reverse transcriptase enhances prime editing by split AAV vectors

ABSTRACT: To investigate if the truncated PE can be dilivered by dual AAV8 vectors for in vivo prime editing. We injected the dual AAV8 into 10-week-old C57BL/6J mice . Livers were isolated 4 weeks after injection and next generation sequencing showed an average of 1.4% and 5.4% precise prime editing with the low and high AAV doses, respectively (Figure 4D ). This demonstrates that PECO-Mini can be efficiently delivered by dual AAVs for in vivo prime editing.

ORGANISM(S): Mus musculus

PROVIDER: GSE205532 | GEO | 2022/06/09

REPOSITORIES: GEO