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Iron-overloaded follicular fluid increases the risk of endometriosis-related infertility by triggering granulosa cell ferroptosis and oocyte dysmaturity [miRNA-seq]

ABSTRACT: To explore the differences of miRNAs from exosomes of granulosa cell in different groups, we extracted exosomes from mouse granulosa cells intervened by different reagents.

ORGANISM(S): Mus musculus

PROVIDER: GSE205579 | GEO | 2022/06/15

REPOSITORIES: GEO

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Iron-overloaded follicular fluid increases the risk of endometriosis-related infertility by triggering granulosa cell ferroptosis and oocyte dysmaturity.

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Iron-overloaded follicular fluid increases the risk of endometriosis-related infertility by triggering granulosa cell ferroptosis and oocyte dysmaturity

Project description:To investigate the different function of endometriosis-related infertile patients follicular fluid (EMFF) and follicular fluid in control group (COFF), follicular fluid was collected and used for RNA sequencing.

2022-06-15 | GSE205494 | GEO

Iron-overloaded follicular fluid increases the risk of endometriosis-related infertility by triggering granulosa cell ferroptosis and oocyte dysmaturity

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Iron-overloaded follicular fluid increases the risk of endometriosis-related infertility by triggering granulosa cell ferroptosis and oocyte dysmaturity [miRNA-seq]

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Project description:Granulosa cells are essential to the growth, development, and maturation of oocytes and follicles. In patients with EMs-related infertility, follicle maturation disorders, poor oocyte quality may be closely related to GCs in the follicle. However, the molecular biological mechanism of GCs in EMs-related infertility has not been reported. Therefore, we tried to explore the possible causes of EMs-related infertility through the study of ovarian GCs in patients with EMs-related infertility and tubal factor infertility.

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granulosa cell tumor vs normal granulosa cells

Project description:mRNA expression profiling in human granulosa cell tumor cell line and normal granulosa cells

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Human Granulosa Cells Proteome

Project description:Human granulosa cells are follicular cells surrounding the oocyte. Human granulosa cells are retrieved during in vitro fertilization a process where patients undergo hormonal stimulation including FSH and LH/hCG stimulation. Under the influence of the luteinizing hormone (LH) a process called luteinization they differentiate to luteal cells and contribute to the corpus luteum. Therefore, this cellular system is a good model for human corpus luteum (CL). To study processes within the human CL, IVF-derived GCs from patients were cultured for two to five days and then analyzed with mass spectrometry based shotgun proteomics.

2019-03-07 | PXD010658 | Pride

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