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Adult Caenorhabditis elegans: wild-type (N2) and mir-243 mutant worms

ABSTRACT: Transcriptional profiling of N2 vs. mir-243 worms, aiming to identify direct and indirect targets of the microRNA.

ORGANISM(S): Caenorhabditis elegans

PROVIDER: GSE20558 | GEO | 2011/09/16

SECONDARY ACCESSION(S): PRJNA125205

REPOSITORIES: GEO

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Adult Caenorhabditis elegans: wild-type (N2) and mir-243 mutant worms

Project description:Transcriptional profiling of N2 vs. mir-243 worms, aiming to identify direct and indirect targets of the microRNA. N2 and mir-243 young-adult worms grown at 20C were analyzed. Three biological replicates with dye-swaps were performed.

2011-09-15 | E-GEOD-20558 | biostudies-arrayexpress

Comparison of gene expression profiles between N2 wild-type C. elegans and an nhr-8(hd117) mutant

Project description:Transcriptional profiling of N2 vs. nhr-8 mutant worms, aiming to identify direct and indirect targets of the nuclear receptor.

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Comparison of gene expression profiles between N2 wild-type C. elegans and an nhr-8(hd117) mutant

Project description:Transcriptional profiling of N2 vs. nhr-8 mutant worms, aiming to identify direct and indirect targets of the nuclear receptor. Seven independent synchronized populations of N2 and nhr-8(hd117) animals were grown from eggs on low cholesterol plates at 25 degrees C until mid-L3 larval stage and collected for microarray RNA expression analysis.

2013-08-06 | E-GEOD-48675 | biostudies-arrayexpress

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Project description:Adult Caenorhabditis elegans: wild-type (N2) and mir-243 mutant worms

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Dynamic gene expression of two Caenorhabditis elegans wild type strains (N2 and CB4856)

Project description:We analyzed the consequences of recombination in gene expression. For this, we measured expression patterns in two C. elegans wild types, N2 and CB4856 (CB). We compare these profiles with gene expression values from a Recombinant Inbred Population (RIL) derived from the same wild types. Expression values of the RILs (GSE17071) were obtained from Viñuela et al. (Genome Research, 2010). N2 and CB strain nematodes were cultured in identical conditions. Likewise, mRNA was extracted at three different ages (juveniles, reproductive and old worms) to investigate the transcriptional consequences of recombination in aging worms. Then, we explored gene expression heritability and transgression as genetic parameters for the analysis of gene expression divergence in natural isolates. Moreover, we investigated the progression of those parameters with age.

2012-04-01 | GSE22887 | GEO

RNA-seq of N2 and HBR227 worms in L1 arrest (48 hours)

Project description:We report the gene expression in L1 arrest (48 hours) of wild-type sleeping worms (N2) and aptf-1(gk794) mutant sleepless worms (HBR227)

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RNA-seq of N2, HBR2340 and PHX2193 worms in L1 arrest (48 hours)

Project description:We report the gene expression in L1 arrest (48 hours) of wild-type sleeping worms (N2) and RIS-manipulated worms (HBR2340 and PHX2193)

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Dynamic gene expression of two Caenorhabditis elegans wild type strains (N2 and CB4856)

Project description:We analyzed the consequences of recombination in gene expression. For this, we measured expression patterns in two C. elegans wild types, N2 and CB4856 (CB). We compare these profiles with gene expression values from a Recombinant Inbred Population (RIL) derived from the same wild types. Expression values of the RILs (GSE17071) were obtained from ViÃ±uela et al. (Genome Research, 2010). N2 and CB strain nematodes were cultured in identical conditions. Likewise, mRNA was extracted at three different ages (juveniles, reproductive and old worms) to investigate the transcriptional consequences of recombination in aging worms. Then, we explored gene expression heritability and transgression as genetic parameters for the analysis of gene expression divergence in natural isolates. Moreover, we investigated the progression of those parameters with age. In total, 14 dual-color microarrays were done on two wild type strains (N2 and CB4856) and three age groups (40, 96, and 214 hours). 5 replicates of t1 (juvenile) and t3 (senescent) samples, and 4 replicates of t2 (reproductive) samples, in a dye-swap design.

2012-03-31 | E-GEOD-22887 | biostudies-arrayexpress

Transcription profiling of C. elegans N2 worms treated with Resveratrol

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