Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression profile at single cell level for induction of human primordial germ cell-like cells (hPGCLCs) in a biomimetic culture

ABSTRACT: Transcriptome at the single-cell level of a biomimetic culture of primed human pluripotent stem cells (hPSCs) was profiled at Day 3 and Day 8. The biomimetic culture, which mimics the peri-implantation human epiblast development, provides an easily implementable culture protocol for deriving human primordial germ cell-like cells (hPGCLCs) from primed hPSCs .

ORGANISM(S): Homo sapiens

PROVIDER: GSE205611 | GEO | 2022/06/10

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

An ExtendedCulture System that Supports Human Primordial Germ Cell-Like Cells Survival and Initiation of DNA Methylation Erasure

Project description:In this study, we evalauted the transcriptionl profiles of human primordial germ cell-like cell (hPGCLCs) cultured for an extended period on STO feeders in FR10 media for an additional 10 days (D4C10). The hPGCLCs were obtained from two different human embryonic stem cell line (hESCs), UCLA 1 (XX) and UCLA2 (XY) for the RNA sequencing data. For whole genome bisulfite sequencing, we evaluate primed UCLA2 hESCs, directly out of the aggragate hPGCLCs (Day 4= D4) and extended culture hPGCLCs D4C10. Our D4C10 hPGCLCs were compared to prior RNA-sequencing data generated in our laboraty, which revealed that D4C10 hPGCLCs retain germline identy and closely resemble D4 hPGCLCs at the transcriptional level. Our WGBS data revealed that extended culture hPGCLCs at D4C10 have a decrease in CG methlyation, as compared to the starting hESCs and D4 hPGCLCs.

2020-01-31 | GSE139115 | GEO

Expanding highly homogenous population of human primordial germ cell like cells in long-term and feeder-free culture condition [WGBS]

Project description:The culture methods for human primordial germ cell like cells (hPGCLCs) has not been established. Here we report the culture method to expand long-term culture hPGCLCs (LTC-hPGCLCs) representing migrating morphology and early hPGC transcriptome and methylome. Key components of the culture media are stem cell factor, STO-conditioned media and absence of fetal calf serum. In this condition, these LTC-hPGCLCs were highly homogenous population without feeder cells and expanded at least 150 days. They did not express senescent phenotype such as β-galactosidase activity and shortening of telomere length at the 150-day culture, indicating that the cell might be a perpetual cell line. LTC-hPGCLCs still have potent pluripotency indicating the phenotype that the cells become human embryonic germ cells which were distinguishable from human iPS cells in strong DLK1 expression and H19 biallelic expression. In summary this simple culture method and highly homogenous LTC-hPGCLCs would be a useful resource that supports investigations on human germline cells.

2021-05-15 | GSE174484 | GEO

Expanding highly homogenous population of human primordial germ cell like cells in long-term and feeder-free culture condition [RNA-Seq]

2021-05-15 | GSE174465 | GEO

Expanding highly homogenous population of human primordial germ cell like cells in long-term and feeder-free culture condition [scRNA-seq]

2021-06-25 | GSE178885 | GEO

Generation of human oogonia from induced pluripotent stem cells in vitro

Project description:Human in vitro gametogenesis may transform reproductive medicine. Human pluripotent stem cells (hPSCs) have been induced into primordial germ cell-like cells (hPGCLCs); however, further differentiation to a mature germ cell has not been achieved. Here, we show that hPGCLCs differentiate progressively into oogonia-like cells during a long-term in vitro culture (approximately 4 months) in xenogeneic reconstituted ovaries with mouse embryonic ovarian somatic cells. The hPGCLC-derived oogonia display hallmarks of epigenetic reprogramming-genome-wide DNA demethylation, imprint erasure, and extinguishment of aberrant DNA methylation in hPSCs-and acquire an immediate precursory state for meiotic recombination. Furthermore, the inactive X chromosome shows a progressive demethylation and reactivation, albeit partially. These findings establish the germline competence of hPSCs and provide a critical step toward human in vitro gametogenesis.

2018-12-18 | GSE117101 | GEO

Gene expression profile at single cell level for induction of human primordial germ cell-like cells (hPGCLCs) in a biomimetic culture

Project description:Gene expression profile at single cell level for induction of human primordial germ cell-like cells (hPGCLCs) in a biomimetic culture

| PRJNA846713 | ENA

A Physiological Biomimetic Culture System for Pig and Human Heart Slices

Project description:A Physiological Biomimetic Culture System for Pig and Human Heart Slices

| PRJNA552335 | ENA

Epigenetic dynamics during capacitation of naïve human pluripotent stem cells [PBAT]

Project description:Human pluripotent stem cells (hPSCs) are of fundamental relevance in regenerative medicine and the primary source for many novel cellular therapies. The development of naïve culture conditions has led to the expectation that these naïve hPSCs could overcome some of the limitations found in conventional (primed) hPSCs culture conditions, including recurrent epigenetic anomalies. Recent work has shown that transition to the primed state (or capacitation) is necessary for naïve hPSCs to acquire multi-lineage differentiation competence. This pluripotent state transition may recapitulate essential features of human peri-implantation development. Here we studied epigenetic changes during the transition between naïve and primed pluripotency, examining global genomic redistribution of histone modifications, chromatin accessibility, and DNA methylation, and correlating these with gene expression. We identify CpG islands, enhancers, and retrotransposons as hotspots of epigenetic dynamics between pluripotency states. Our results further reveal that hPSC resetting and subsequent capacitation rescue X chromosome-linked epigenetic erosion and reduce the ectoderm-biased gene expression of conventional primed hPSCs.

2023-10-03 | GSE218511 | GEO

Epigenetic dynamics during capacitation of naïve human pluripotent stem cells [ChIP-seq]

2023-10-03 | GSE218510 | GEO

Epigenetic dynamics during capacitation of naïve human pluripotent stem cells [ATAC-seq]

2023-10-03 | GSE218509 | GEO