Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Psoriatic arthritis skin lesions and uninvolved bulk RNA-seq

ABSTRACT: Investigation of the transcriptomic differences that occur in psoriatic skin lesions, compared to non arthritic skin or non-lesion arthritic skin.

ORGANISM(S): Homo sapiens

PROVIDER: GSE205748 | GEO | 2023/05/10

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Psoriatic arthritis skin lesions and uninvolved bulk RNA-seq

Project description:Psoriatic arthritis skin lesions and uninvolved bulk RNA-seq

| PRJNA847432 | ENA

Global methylation analysis of psoriatic (involved and uninvolved) and control skin

Project description:Psoriasis is a chronic inflammatory immune-mediated disorder affecting the skin and other organs including joints. Over 1,300 transcripts are altered in psoriatic involved skin compared to normal skin. Global epigenetic profiles of psoriatic skin have not been described. Here we describe the first genome-wide study of altered CpG methylation in psoriatic skin. We determined the methylation levels at 27,578 CpG sites in skin samples from individuals with psoriasis (12 involved, 8 uninvolved) and 10 unaffected individuals. CpG methylation of involved skin differed from normal skin at 1,108 sites. Twelve mapped to the epidermal differentiation complex, upstream or within genes that are highly up-regulated in psoriasis. Hierarchical clustering of 50 of the top differentially methylated (DM) sites separated psoriatic from normal skin samples. CpG sites where methylation was correlated with gene expression are reported. Sites with inverse correlations between methylation and nearby gene expression include those of KYNU, OAS2, S100A12, and SERPINB3, whose strong transcriptional up-regulation are important discriminators of psoriasis. We observed intrinsic epigenetic differences in uninvolved skin. Pyrosequencing of bisulfite-treated DNA from skin biopsies at three DM loci confirmed earlier findings and revealed a reversion of methylation levels towards the non-psoriatic state after one month of anti-TNF-a therapy.

2011-11-11 | GSE31835 | GEO

Global methylation analysis of psoriatic (involved and uninvolved) and control skin

2011-11-11 | E-GEOD-31835 | biostudies-arrayexpress

Gene expression profiling in psoriatic lesional and non-lesional skin [Set 1]

Project description:To explore the psoriasis phenotype, we characterize gene expression in lesional and non-lesional skin from psoriasis patients. We extracted total RNA from 5mm punch biopsies taken from 14 psoriatic patients. From each patient, we obtained two biopsies, one from a lesion and the other from non-lesional skin in the same general body geography. A total of 28 samples were run on Affymetrix HU133 Plus 2.0 microarrays.

2013-01-28 | E-GEOD-34248 | biostudies-arrayexpress

Identifying Serum Metabolomic Markers Associated with Skin Disease Activity in patients with Psoriatic Arthritis

Project description:This dataset contains .RAW files acquired for the paper: Identifying Serum Metabolomic Markers Associated with Skin Disease Activity in patients with Psoriatic Arthritis.

| MSV000092967 | MassIVE

Transcriptome analysis of subcutaneous adipose tissue from psoriatic lesion skin and peripheral non-lesional skin

Project description:Transcriptome analysis of subcutaneous adipose tissue from psoriatic lesion skin and peripheral non-lesional skin

| PRJNA1160898 | ENA

Laser capture microdissection followed by next-generation sequencing identifies disease-related microRNAs in psoriatic skin that mirror the systemic psoriatic miRNome

Project description:Psoriasis is a systemic disease with cutaneous manifestations. MicroRNAs (miRNAs) are non-coding RNA molecules that are differentially expressed in psoriatic skin, however; only few miRNAs have been localized to specific cells or regions of psoriatic lesions. We used laser capture microdissection (LCM) and next-generation sequencing to study the specific miRNA expression profiles in the epidermis (Epi) and dermal inflammatory aggregates (RD/ICs) of psoriatic skin. We identified 24 deregulated miRNAs in the Epi and 37 deregulated miRNAs in the RD/ICs of lesional psoriatic skin compared with non-lesional psoriatic skin (FCH>2, FDR<0.05). Interestingly, 9 of the 37 miRNAs, including miR-193b and miR-223 that have recently been described as deregulated in circulating peripheral blood mononuclear cells (PBMCs) from patients with psoriasis. Using flow cytometry and qRT-PCR, miR-193b and miR-223 were found to be expressed in Th17 cells. In conclusion, we demonstrate that LCM combined with small RNA sequencing provides a robust strategy to explore the global miRNA expression in the epidermal and dermal compartments of psoriatic skin. Furthermore, our results indicate that the altered local miRNA changes seen in the RD/ICs is reflected in the circulating immune cells, altogether emphasizing that miRNAs may contribute to a systemic component in the pathogenesis of psoriasis. Examination of the global miRNA expression in epidermis (Epi) and dermis (RD/ICs) of paired (non-lesional vs. lesional) psoriatic skin using a combination of laser-capture microdissection and barcoded small RNA sequencing

2015-04-01 | E-GEOD-57012 | biostudies-arrayexpress

The Characteristics of cell in Psoriatic Resolved Lesions Revealed and Onsite Recurrent by Single-cell Transcriptome

Project description:Skin biopsy from the previously resolved region (PN), the autologous on-site recurrence with psoriatic plaque (PP)and the non-lesion control (CN) were obtained from the lower back of four male plaque psoriasis patients. Single-cell RNA sequencing (scRNA-seq) was performed after cell dissociation. In our article, sub-clustering and characterization of T cells were demonstrated, functional comparisons of T-cell subsets were conducted further among PN, PP and CN.

2024-07-29 | GSE248121 | GEO

Small RNA sequencing in normal and psoriatic skin

Project description:We report the application of Illumina small RNA sequencing to normal human skin, as well as uninvolved and involved psoriatic skin. By obtaining over 600 million qualified reads from 20 healthy controls and 47 psoriasis biopsies (uninvolved/involved), we have generated a complete small RNA profile in normal and diseased human skin, with particular emphasis on miRNAs. We report the discovery of 284 putative novel miRNAs as well as 98 differentially expressed miRNAs in psoriatic skin. miRNA discovery and expression profiling in 67 normal and psoriatic human skin biopsies

2011-08-19 | E-GEOD-31037 | biostudies-arrayexpress

Transcriptome analysis of subcutaneous adipose tissue from psoriatic lesion skin and peripheral non-lesional skin

Project description:Psoriasis, a prevalent chronic inflammatory skin disease, affects over 100 million individuals globally and is increasingly acknowledged as a systemic condition impacting the heart, blood vessels, and joints. A substantial body of research highlights a strong correlation between the severity of psoriasis and an elevated risk of cardiovascular diseases. Notably, treatments that alleviate dermatological symptoms have also been shown to reduce the formation of coronary plaques. Adipose tissue, functioning as a significant endocrine organ, secretes pro-inflammatory mediators that perpetuate the chronic low-grade inflammation typical of obesity. To investigate the molecular changes in adipose tissue associated with psoriasis, we collected subcutaneous adipose tissue samples from both psoriatic lesion skin (LS, n=8) and peripheral non-lesional skin (NLS, n=8), and conducted comprehensive transcriptomic analyses.

2025-06-07 | GSE277173 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data