RNA-seq of single cholangiocyte-derived organoids reveals high organoid-to-organoid variability

ABSTRACT: Organoids have been established from the majority of tissue resident stem cells. In recent years, several reports hinted at intraculture organoid variability, but a systematic analysis of such a heterogeneity has not been performed before. Here, we used RNA-seq of individual organoids to address this question. Importantly, we find that batch-to-batch variation is very low, even when prepared by different researchers. On the other hand, there is organoid-to-organoid variability within a culture. Using differential gene expression, we did not identify specific pathways that drive this variability, pointing towards possible effects of the microenvironment within the culture condition.

ORGANISM(S): Mus musculus

PROVIDER: GSE205753 | GEO | 2022/08/01

REPOSITORIES: GEO