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Generation of mesenchymal stem/stromal cells (MSCs) from human iPSCs via neural crest cells (NCCs)

ABSTRACT: To clarify the changes in cell properties during the differentiation process from iPSCs into MSC through NCCs, we sampled each cell during the differentiation process and compared gene expression patterns.

ORGANISM(S): Homo sapiens

PROVIDER: GSE206128 | GEO | 2022/06/19

REPOSITORIES: GEO

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Time course expression pattern during neural crest cells (NCCs) differentiation from iPSCs

Project description:To clarify the changes in cell properties during the differentiation process from iPSCs into NCCs, we sampled each cell during the differentiation process and compared gene expression patterns.

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Generation of mesenchymal stem/stromal cells (MSCs) from human iPSCs via neural crest cells (NCCs)

Project description:Generation of mesenchymal stem/stromal cells (MSCs) from human iPSCs via neural crest cells (NCCs)

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2015-09-17 | E-GEOD-61510 | biostudies-arrayexpress

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Pioneering function of Yap and Taz in promoting osteogenesis of neural crest cells by preventing chondrogenesis

Project description:Neural crest cells (NCCs) are multipotent stem cells with a remarkable ability to differentiate into multiple cell lineages, including osteoblasts and chondrocytes. NCCs contribute to the majority of craniofacial skeleton, yet the molecular mechanisms regulating NCCs diversification into osteoblasts or chondrocytes remain poorly understood. We found that Yap and Taz function redundantly as key determinants of the osteogenesis versus chondrogenesis fate decision and differentiation in NCCs in vitro, ex vivo and in vivo, and Yap/Taz-deficiency in NCCs resulted in a switch from osteogenesis to chondrogenesis. Comprehensive analysis of unbiased datasets including CUT&RUN-seq and RNA-seq indicated that Yap/Taz directly regulate key genes that govern osteogenesis and chondrogenesis. During NCC-derived osteogenesis, Yap/Taz promote expression of osteogenic genes such as Runx2 and Sp7 but repress expression of chondrogenic genes such as Sox9 and Col2a1. Further, we found Yap/Taz directly interact with β-catenin in NCCs to coordinately promote osteoblast differentiation and repress chondrogenesis. Together our data indicate that Yap/Taz promote osteogenesis in NCCs by preventing chondrogenesis, partly through interactions with the Wnt-β-catenin pathway.

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P0-cre marked neural crest cells in murine embryo

Project description:To elucidate the molecular features of craniofacial versus trunk neural crest cells (NCCs), we utilized P0-Cre/Floxed-EGFP mice that specifically label NCCs (Yamauchi et al., 1999 (PMID 10419695)). Craniofacial and trunk regions were isolated from P0-Cre/Floxed-EGFP mouse embryos at embryonic day E12.5, and dissociated cells were analyzed by flow cytometory in regard to the intensity of EGFP. In this study, we performed at least duplicate experiments for each of the four groups (Craniofacial EGFP+, Trunk EGFP+, Craniofacial EGFP-, Trunk EGFP-). Total of 9 samples.

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