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Identification of smRNA-based cell-type markers for the stem cell manufacture system with a statistical scoring function

ABSTRACT: Cell-type biomarkers are useful in stem cell manufacture to monitor cell purification, cell quantity, and quality. However, the study on cell-type marker, specifically for stem cell manufacture, is limited. The emerging questions are which RNA transcripts can serve as biomarkers during stem cell culture, and what method can efficiently and accurately discover these biomarkers. We developed a scoring function system to identify small RNA (smRNA) biomarkers with RNA-seq data. We applied the method to two data sets, one for extracellular smRNAs and the other for intracellular smRNAs. To get the RNA-seq data from intracellular smRNAs, we cultured four types of cells: human embryo stem cells (H9), neural stem cells (NSC), hESC-derived endothelial cells (EC) and conducted small RNA-seq to their intracellular smRNAs. Using these data, we identified a set of smRNAs as candidates of biomarkers for different types of cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE206925 | GEO | 2022/08/01

REPOSITORIES: GEO

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