Genomics

Dataset Information

0

A miRNA signature for early non-invasive diagnosis of pre-eclampsia


ABSTRACT: Background: Preeclampsia (PE) is a multi-systemic maternal syndrome with substantial maternal and fetal morbidity and mortality. Currently, there is no clinically viable non-invasive biomarker assay for early detection, thus limiting the effective prevention and therapeutic strategies for PE. Methods: We conducted a discovery-training-validation three-phase retrospective and prospective study with cross-platform and multicenter cohorts. The initial biomarkers were discovered and verified in tissue specimens by small RNA sequencing and qRT-PCR. A miRNA signature (miR2PE-score) was developed using the Firth’s bias-reduced logistic regression analysis, and subsequently validated in two independent multinational retrospective cohorts and two prospective plasma cohorts. Results: We initially identified five PE-associated differentially expressed miRNAs from miRNA sequencing data and subsequently validated two miRNAs (miR-196b-5p and miR-584-5p) as robust biomarkers by association analysis with clinical characteristics and qRT-PCR in tissue specimens in the discovery phase. Using the Firth’s bias-reduced logistic regression analysis, we developed the miR2PE-score for the early detection of PE. The miR2PE-score showed a high diagnostic performance with an area under the receiver operating characteristic curve (AUROC) of 0.920, 0.848, 0.864 and 0.812 in training, internal and two external validation cross-platform and multicenter cohorts, respectively. Finally, we demonstrated the non-invasive diagnostic performance of the miR2PE-score in two prospective plasma cohorts with AUROC of 0.933 and 0.787. Furthermore, the miR2PE-score revealed superior performance in non-invasive diagnosis compared with previously published miRNA biomarkers. Conclusions: We developed and validated a novel and robust blood-based miRNA signature, which may serve as a promising clinically applicable non-invasive tool for the early detection of PE.

ORGANISM(S): Homo sapiens

PROVIDER: GSE206988 | GEO | 2022/09/21

REPOSITORIES: GEO

Similar Datasets

2022-03-03 | PXD029009 | Pride
2020-11-15 | GSE140494 | GEO
2023-12-22 | GSE251855 | GEO
2018-02-18 | GSE95246 | GEO
2019-02-26 | GSE120584 | GEO
2022-02-21 | GSE197020 | GEO
2015-05-17 | E-GEOD-66499 | biostudies-arrayexpress
2023-06-14 | GSE234611 | GEO
2021-12-29 | GSE175648 | GEO
2023-06-20 | GSE235067 | GEO