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CBP and Gcn5 drive zygotic genome activation in the Drosophila embryo

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE207019 | GEO | 2023/04/26

REPOSITORIES: GEO

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CBP and Gcn5 drive zygotic genome activation independently of their catalytic activity.

Project description:Zygotic genome activation (ZGA) is a crucial step of embryonic development. So far, little is known about the role of chromatin factors during this process. Here, we used an in vivo RNA interference reverse genetic screen to identify chromatin factors necessary for embryonic development in Drosophila melanogaster. Our screen reveals that histone acetyltransferases (HATs) and histone deacetylases are crucial ZGA regulators. We demonstrate that Nejire (CBP/EP300 ortholog) is essential for the acetylation of histone H3 lysine-18 and lysine-27, whereas Gcn5 (GCN5/PCAF ortholog) for lysine-9 of H3 at ZGA, with these marks being enriched at all actively transcribed genes. Nonetheless, these HATs activate distinct sets of genes. Unexpectedly, individual catalytic dead mutants of either Nejire or Gcn5 can activate zygotic transcription (ZGA) and transactivate a reporter gene in vitro. Together, our data identify Nejire and Gcn5 as key regulators of ZGA.

| S-EPMC10121174 | biostudies-literature

CBP and Gcn5 drive zygotic genome activation in the Drosophila embryo [CUT&Tag]

Project description:Mapping H3K9ac, H3K18ac and H3K27ac during Drosophila zygotic genome activation Mapping Gcn5 and Nejire in catalytic wild-type or catalytic dead rescue of Gcn5KD and NejireKD during Drosophila zygotic genome activation

2023-04-26 | GSE207016 | GEO

CBP and Gcn5 drive zygotic genome activation in the Drosophila embryo

Project description:CBP and Gcn5 drive zygotic genome activation in the Drosophila embryo

| PRJNA853320 | ENA

CBP and Gcn5 drive zygotic genome activation in the Drosophila embryo [CUT&Tag]

Project description:CBP and Gcn5 drive zygotic genome activation in the Drosophila embryo [CUT&Tag]

| PRJNA853325 | ENA

Unmasking activation of the zygotic genome using chromosomal deletions in the Drosophila embryo.

Project description:During the maternal-to-zygotic transition, a developing embryo integrates post-transcriptional regulation of maternal mRNAs with transcriptional activation of its own genome. By combining chromosomal ablation in Drosophila with microarray analysis, we characterized the basis of this integration. We show that the expression profile for at least one third of zygotically active genes is coupled to the concomitant degradation of the corresponding maternal mRNAs. The embryo uses transcription and degradation to generate localized patterns of expression, and zygotic transcription to degrade distinct classes of maternal transcripts. Although degradation does not appear to involve a simple regulatory code, the activation of the zygotic genome starts from intronless genes sharing a common cis-element. This cis-element interacts with a single protein, the Bicoid stability factor, and acts as a potent enhancer capable of timing the activity of an exogenous transactivator. We propose that this regulatory mode links morphogen gradients with temporal regulation during the maternal-to-zygotic transition.

| S-EPMC1854917 | biostudies-literature

GAF is essential for zygotic genome activation and chromatin accessibility in the early Drosophila embryo

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-03-03 | GSE152773 | GEO

Correction: Unmasking Activation of the Zygotic Genome Using Chromosomal Deletions in the Drosophila Embryo

Project description: Not available

| S-EPMC1945081 | biostudies-literature

GAF is essential for zygotic genome activation and chromatin accessibility in the early Drosophila embryo.

Project description:Following fertilization, the genomes of the germ cells are reprogrammed to form the totipotent embryo. Pioneer transcription factors are essential for remodeling the chromatin and driving the initial wave of zygotic gene expression. In Drosophila melanogaster, the pioneer factor Zelda is essential for development through this dramatic period of reprogramming, known as the maternal-to-zygotic transition (MZT). However, it was unknown whether additional pioneer factors were required for this transition. We identified an additional maternally encoded factor required for development through the MZT, GAGA Factor (GAF). GAF is necessary to activate widespread zygotic transcription and to remodel the chromatin accessibility landscape. We demonstrated that Zelda preferentially controls expression of the earliest transcribed genes, while genes expressed during widespread activation are predominantly dependent on GAF. Thus, progression through the MZT requires coordination of multiple pioneer-like factors, and we propose that as development proceeds control is gradually transferred from Zelda to GAF.

| S-EPMC8079149 | biostudies-literature

CLAMP regulates zygotic genome activation in Drosophila embryos.

Project description:Embryonic patterning is critically dependent on zygotic genome activation (ZGA). In Drosophila melanogaster embryos, the pioneer factor Zelda directs ZGA, possibly in conjunction with other factors. Here, we have explored the novel involvement of Chromatin-Linked Adapter for MSL Proteins (CLAMP) during ZGA. CLAMP binds thousands of sites genome-wide throughout early embryogenesis. Interestingly, CLAMP relocates to target promoter sequences across the genome when ZGA is initiated. Although there is a considerable overlap between CLAMP and Zelda binding sites, the proteins display distinct temporal dynamics. To assess whether CLAMP occupancy affects gene expression, we analyzed transcriptomes of embryos zygotically compromised for either clamp or zelda and found that transcript levels of many zygotically activated genes are similarly affected. Importantly, compromising either clamp or zelda disrupted the expression of critical segmentation and sex determination genes bound by CLAMP (and Zelda). Furthermore, clamp knockdown embryos recapitulate other phenotypes observed in Zelda-depleted embryos, including nuclear division defects, centrosome aberrations, and a disorganized actomyosin network. Based on these data, we propose that CLAMP acts in concert with Zelda to regulate early zygotic transcription.

| S-EPMC8633140 | biostudies-literature

Spatiotemporal Patterning of Zygotic Genome Activation in a Model Vertebrate Embryo.

Project description:A defining feature of early embryogenesis is the transition from maternal to zygotic control. This transition requires embryo-wide zygotic genome activation (ZGA), but the extent of spatiotemporal coordination of ZGA between individual cells is unknown. Multiple interrelated parameters, including elapsed time, completed cycles of cell division, and cell size may impact ZGA onset; however, the principal determinant of ZGA during vertebrate embryogenesis is debated. Here, we perform single-cell imaging of large-scale ZGA in whole-mount Xenopus embryos. We find a striking new spatiotemporal pattern of ZGA whose onset tightly correlates with cell size but not with elapsed time or number of cell divisions. Further, reducing cell size induces premature ZGA, dose dependently. We conclude that large-scale ZGA is not spatially uniform and that its onset is determined at the single-cell level, primarily by cell size. Our study suggests that spatial patterns of ZGA onset may be a common feature of embryonic systems.

| S-EPMC6655562 | biostudies-literature

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