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Keratin15 Regulates Cell Proliferation in Outer Enamel Epithelium

ABSTRACT: The keratins are the typical intermediate filament proteins of epithelia, presenting with highly specific expression patterns related to the epithelial type and stage of cellular differentiation. They are important for the cytoplasmic stability and integrity of epithelia, and also involved in various intracellular signaling pathways. Several keratins have been proved to be associated with enamel formation. However, there is still lack of information on the expression patterns of keratins during tooth morphogenesis. In the present study, we analyzed the spatiotemporal expression of the keratin family members using cap analysis gene expression and single-cell RNA-sequence analysis, revealing that Krt15 and Krt17 were not only highly expressed during tooth development, but also specifically expressed in non-ameloblasts. Immunostaining of both embryotic and postnatal mouse tooth germs showed that keratin 15 was mostly expressed in outer enamel epithelium, whereas keratin 17 was mainly found in stratum intermedium. Microarray analysis discovered that a core set of proliferation related genes were upregulated in Krt15-knockdown dental epithelial cells. The results of both CCK8 assay and EdU staining indicated that the loss of keratin 15 resulted in a higher cell proliferation activity. Our findings suggested that Krt15 could be a marker gene of outer enamel epithelium, and might take a part in regulating cell proliferation of dental epithelial cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE207156 | GEO | 2025/06/24

REPOSITORIES: GEO

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