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Profiling the intragenic toxicity determinants of the hok/Sok toxin-antitoxin system

ABSTRACT: Here, we adapted and improved our FASTBAC-Seq method originally designed in Helicobacter pylori to investigate T1TAs in the model organism Escherichia coli. Our approach combines a life and death selection with deep-sequencing to assess the killing capability of a toxin and obtain an overview of single-nucleotide substitutions suppressing the toxin expression or activity in absence of its antitoxin. As a proof of concept, we revisited the regulation of the plasmidic hok/Sok T1TA system.

ORGANISM(S): Escherichia coli

PROVIDER: GSE207195 | GEO | 2022/10/06

REPOSITORIES: GEO

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