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Regulatory variants in a novel distal enhancer regulate the expression of CYP3A4 and CYP3A5: ATAC-Seq and 4C data from human hepatocytes [ATAC-seq]

ABSTRACT: The cytochrome P450 3As (CYP3As) are abundantly expressed in the liver and metabolize many commonly prescribed medications. Their expression is highly variable between individuals with little known genetic cause. Despite extensive investigation, cis-acting genetic elements that control the expression of CYP3As remain uncharacterized. Using chromatin conformation capture (4C assays), we detected reciprocal interaction between a distal regulatory region (DDR) and the CYP3A4 promoter. The DRR colocalizes with a variety of enhancer marks and was found to promote transcription in reporter assays. CRISPR-mediated knockout of the DRR decreased expression of CYP3A4, CYP3A5, and CYP3A7, supporting its role as a shared enhancer regulating the expression of three CYP3A genes. Here we provided data generated from ATAC-Seq analysis of human primary hepatocytes originating from both black and white donors.

ORGANISM(S): Homo sapiens

PROVIDER: GSE207590 | GEO | 2023/06/26

REPOSITORIES: GEO

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Regulatory variants in a novel distal enhancer regulate the expression of CYP3A4 and CYP3A5: ATAC-Seq and 4C data from human hepatocytes [4C]

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