Transcriptome analysis of Fusarium solani (NK-NH1) before and after inhibition of volatile organic compounds produced by Xenorhabdus bovienii
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Illumina HiSeq 2500 (Fusarium solani)
ORGANISM(S): Fusarium Solani
SUBMITTER: 
Yu Wang
PROVIDER: GSE207994 | GEO | 2022-07-15
REPOSITORIES: GEO
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Project description:The aims of this work were to screen isolated bacteria with a dual capacity: to inhibit <i>Fusarium solani</i> and to promote plant growth. Also, volatile compounds that would be responsible for that effect were identified. Seventy bacterial strains from the air, agricultural soils, hydrocarbons-contaminated soils, and extremophile soils were tested. The former were identified by Matrix-Assisted Laser Desorption/Ionization-time of flight mass spectrometry and 16S rDNA sequencing. The plant growth-promoting bacteria (PGPB) and their capability for phosphate solubilization, siderophores production, and indole production were determined. Twenty isolates from <i>Bacillus</i> and <i>Pseudomonas</i> genera inhibited the mycelial growth up to 40% in direct assays. Eleven isolates significantly inhibited mycelial growth in 18-24% via volatile emissions. Volatile compounds related to antifungal activity or stress response include ketones, sesquiterpenes, monoterpenoids, alkanes, and fatty acids. Our results support the potential of these PGPB to act as biocontrol agents against fungal pathogens via volatile emissions.
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Project description:The antagonistic mechanisms of soluble non-volatile bioactive compounds, such as proteins and lipopeptides emitted from <i>Bacillus</i> have been widely studied. However, there are limited studies on the antifungal mechanisms of volatile organic compounds (VOCs) produced by <i>Bacillus</i> against plant fungal diseases. In this study, the antagonistic mechanisms of one specific VOC, 6-methyl-2-heptanone, against <i>Alternaria solani</i> were investigated. To optimize the extraction conditions of headspace solid-phase microextraction, a 50/30-μm divinylbenzene/carboxen/polydimethylsiloxane fiber at 50°C for 40 min was used. For gas chromatography-mass spectrometry using a free fatty acid phase capillary column, 6-methyl-2-heptanone accounted for the highest content, at 22.27%, of the total VOCs from <i>Bacillus subtilis</i> ZD01, which inhibited <i>A. solani</i> mycelial growth strongly <i>in vitro</i>. Therefore, 6-methyl-2-heptanone was selected as the main active chemical to elucidate the action mechanisms against <i>A. solani</i>. Scanning and transmission electron microscopy analyses revealed that after exposure to an EC<sub>50</sub> dose of 6-methyl-2-heptanone, <i>A. solani</i> hyphal cells had a wide range of abnormalities. 6-Methyl-2-heptanone also caused the capture of cellular fluorescent green label and the release of adenosine triphosphate (ATP) from outer membranes <i>A. solani</i> cells, which may enhance 6-methyl-2-heptanone ability to reach the cytoplasmic membrane. In addition, 6-methyl-2-heptanone showed strong inhibitory effect on <i>A. solani</i> conidial germination. It also damaged conidial internal structures, with the treated group having collapsed shrunken small vesicles as observed by transmission electron microscopy. Because 6-methyl-2-heptanone showed strong effects on mycelial integrity and conidial structure, the expression levels of related pathogenic genes in <i>A. solani</i> treated with 6-methyl-2-heptanone were investigated. The qRT-PCR results showed that transcriptional expression levels of <i>slt2</i> and <i>wetA</i> genes were strongly down-regulated after exposure to 6-methyl-2-heptanone. Finally, because identifying the functions of pathogenic genes will be important for the biological control of <i>A. solani</i>, the <i>wetA</i> gene was identified as a conidia-associated gene that plays roles in regulating sporulation yield and conidial maturation. These findings provide further insights into the mechanisms of VOCs secreted by <i>Bacillus</i> against <i>A. solani</i>.
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Project description:Oomycetes, represented by <i>Phytophthora</i>, are seriously harmful to agricultural production, resulting in a decline in grain quality and agricultural products and causing great economic losses. Integrated management of oomycete diseases is becoming more challenging, and plant derivatives represent effective alternatives to synthetic chemicals as novel crop protection solutions. Biologically active secondary metabolites are rapidly synthesized and released by plants in response to biotic stress caused by herbivores or insects, as well as pathogens. In this study, we identified groups of volatile organic compounds (VOCs) from soybean plants inoculated with <i>Phytophthora sojae</i>, the causal agent of soybean root rot. 4-Ethylphenol was present among the identified VOCs and was induced in the incompatible interaction between the plants and the pathogen. 4-Ethylphenol inhibited the growth of <i>P. sojae</i> and <i>Phytophthora nicotianae</i> and had toxicity to sporangia formation and zoospore germination by destroying the pathogen cell membrane; it had a good control effect on soybean root rot and tobacco black shank in the safe concentration range. Furthermore, 4-Ethylphenol had a potent antifungal activity against three soil-borne phytopathogenic fungi, <i>Rhizoctonia solani</i>, <i>Fusarium graminearum</i>, and <i>Gaeumannomyces graminis</i> var <i>tritici</i>, and four forma specialis of <i>Fusarium oxysporum</i>, which suggest a potential to be an eco-friendly biological control agent.
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Project description:Verticillium wilt of cotton, caused by the soilborne pathogen Verticillium dahliae, is one of the most serious diseases of cotton worldwide. Increased concerns about the side effects of synthetic pesticides have resulted in greater interest in developing biocontrol strategies against Verticillium wilt. We evaluated a Fusarium solani CEF559 isolate, obtained from the endosphere of healthy cotton plants, for its biocontrol potential against V. dahliae in vitro and in vivo. In addition to disease assessment, three key genes in the lignin metabolism pathway and four pathogenesis-related (PR) genes were monitored using qRT-PCR. In the laboratory tests, F. solani CEF559 inhibited V. dahliae colony growth by 75% and sporulation by nearly 80% and completely suppressed conidial production. However, volatile metabolites from CEF559 did not affect V. dahliae colony growth. In the greenhouse study, CEF559 significantly reduced wilt development, with a control efficacy greater than 60% when assessed 25 days postinoculation. In a field experiment, CEF559 reduced wilt development, with the efficacy ranting from 30.1% to 56.3%. PR genes and those key genes in the lignin metabolism pathway were transiently upregulated in the cotton roots pretreated with CEF559 when subsequently inoculated with V. dahliae, compared with those plants inoculated with V. dahliae only. Moreover, CEF559 inhibited V. dahliae colonisation of both the roots and hypocotyls. The present results suggest that this cotton endophytic fungal strain, F. solani CEF559, confers protection against V. dahliae.
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Project description:The plant-growth modulating effect of microbial volatile organic compounds (VOCs) has been demonstrated repeatedly. This has most often been performed by exposing plants to VOC released by microbes grown on nutrient rich media. Here, we used soil instead to grow fungi of the Fusarium genus and investigate how VOCs emitted by this system influenced the development of Arabidopsis plants. The volatile profiles of Fusarium strains grown in soil and malt extract were also compared. Our results demonstrate that distinct volatile signatures can be attributed to different Fusarium genetic clades but also highlight a major influence of the growth medium on volatile emission. Furthermore, all soil-grown Fusarium isolates increased primary root length in Arabidopsis by decreasing VOC concentrations in soil. This result represents a major paradigm shift in plant-microbe interactions since growth modulating effects have been attributed so far to the emission and not the consumption of volatile signals.
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Project description:Context: Nigerian soil fungi population is unexplored. It is hypothesized that they harbour new bioactive chemicals. This hypothesis is based on the large percentage of currently approved medicines that originated from soil-inhabiting micro-organisms Objectives: To investigate the antimicrobial and anti-inflammatory properties of Fusarium solani ethyl acetate (EtOAc) extract selected based on its broad spectrum of antimicrobial potential in an overlay experiment with seven other soil fungi strains. Materials and methods: Fungus number 6 (F6), identified by molecular characterization as Fusarium solani (Mart.) Sacc (Nectriaceae) was selected for studies from eight purified soil fungi due to its superior broad-spectrum antibiotics producing potential following agar overlay experiment. F6 was fermented for 21?d and the minimum inhibitory concentration (MIC) of its EtOAc fermentation extract (dose range: 12.5-100?µg/mL) was determined using agar dilution method for Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli, Salmonella typhi and anti-inflammatory properties determined using rat-paw (250-500?mg/kg) and xylene induced oedema (250-500?µg/kg) (in Swiss albino rats and mice) models, respectively. The ability of the extract to inhibit cyclooxygenase (COX) enzyme was also determined in vitro using Cayman test kit-760111. Result: The MIC of the EtOAc extract was <12.5?µg/mL for S. aureus, P. aeruginosa and Escherichia coli. It inhibited xylene induced oedema by 65% compared with 61% observed for diclofenac and was significantly (p?<?0.05) better than diclofenac in rat-paw-oedema model within the first phase of inflammation. The extract inhibited COX-1 peroxidase-mediated activities with an IC50 below 5?µg/mL. Conclusions: The extract exhibited strong antibacterial and anti-inflammatory properties, warranting further investigations into therapeutic potential of this fungus. This study design can be adapted in soil fungi metabolomic investigations. We report for the first time the potent anti-inflammatory property of the ethyl acetate extract of soil strain of F. solani with a possible mechanism of action that involves the inhibition of COX enzyme.
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Project description:Rhizobacteria emit bioactive metabolites with antifungal properties that could be used for biocontrol of fungal diseases. In this study, we evaluated the potential of diffusible and volatile organic compounds (VOCs) emitted by avocado rhizobacteria to inhibit the growth of Fusarium kuroshium, one of the causal agents of Fusarium dieback (FD) in avocado. Three bacterial isolates (INECOL-6004, INECOL-6005, and INECOL-6006), belonging to the Bacillus genus, were selected based on their capacity to inhibit several avocado fungal pathogens, and tested in antagonism assays against F. kuroshium. The three bacterial isolates significantly inhibited F. kuroshium mycelial growth by up to 48%. The composition of bacterial diffusible compounds was characterized by the analysis of EtOAc and n-BuOH extracts by using ultra-performance liquid chromatography (UPLC) coupled to high-resolution mass spectrometry (HRMS). The three bacterial isolates produced cyclo-lipopeptides belonging to the iturin, fengycin, and surfactin families. The antifungal activity of n-BuOH extracts was larger than that of EtOAc extracts, probably due to the greater relative abundance of fengycin in the former than in the latter. In addition, isolates INECOL-6004 and INECOL-6006 significantly inhibited F. kuroshium mycelial growth through VOC emission by up to 69.88%. The analysis of their VOC profiles by solid phase micro-extraction (SPME) coupled to gas chromatography and mass spectrometry (GC-MS) revealed the presence of ketones and pyrazine compounds, particularly of 2-nonanone, which was not detected in the VOC profile of isolate INECOL-6005. These results emphasize the need to further investigate the antifungal activity of each bioactive compound for the development of new formulations against fungal phytopathogens.
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