Accurate simultaneous sequencing of genetic and epigenetic bases in DNA

ABSTRACT: We present a single-base-resolution sequencing methodology that will simultaneously sequence complete genetics and complete epigenetics in a single workflow. The approach is non-destructive to DNA and provides a digital readout of bases, which we exemplify by simultaneous sequencing of G, C, T, A, and 5mC/5hmC (5-letter sequencing) or 5mC and 5hmC (6-Letter sequencing). We demonstrate sequencing of human genomic DNA and also cell-free DNA taken from a blood sample of a cancer patient. The approach is accurate, requires low DNA input and has a simple workflow and analysis pipeline. We envisage it will be versatile across many applications in life sciences.

ORGANISM(S): Homo sapiens

PROVIDER: GSE208549 | GEO | 2022/07/24

REPOSITORIES: GEO