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Differential expression analysis of Thermus thermophilus HB8 wild-type strain in response to zinc

ABSTRACT: We observed the expression profile of the total mRNA in Thermus thermophilus HB8 wild-type strain at 0 and 30 min after the addition of ZnSO4.

ORGANISM(S): Thermus thermophilus HB8

PROVIDER: GSE20900 | GEO | 2010/04/13

SECONDARY ACCESSION(S): PRJNA124399

REPOSITORIES: GEO

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Differential expression analysis of Thermus thermophilus HB8 wild-type strain in response to zinc

Project description:We observed the expression profile of the total mRNA in Thermus thermophilus HB8 wild-type strain at 0 and 30 min after the addition of ZnSO4. Three wild-type strain of T. thermophilus HB8 were pre-cultured at 70 degC for 16 hours in 3 mL of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (1 mL) were inoculated into 250 mL of the synthetic medium and then cultivated at 70°C for 8 hours (A600 value being ~0.52). Then the 1.0 mM of ZnSO4 was added into the medium, and continued cultivation. Cells were collected at 0 and 30 min time point after the addition of zinc ion, and then crude RNA was extracted. The expression of each mRNA at each time point was analyzed on a GeneChip as described under Sample Description Sheet of each sample.

2010-04-13 | E-GEOD-20900 | biostudies-arrayexpress

Expression profile of crp-deficient Thermus thermophilus HB8 strain

Project description:We observed the expression profile of the total mRNA of crp (TTHA1437) deletion mutant of Thermus thermophilus HB8 strain grown in a rich (TT) medium at 70 degC

2010-10-15 | GSE21459 | GEO

TTHB212 (anti-sigmaE)-deficient Thermus thermophilus HB8 strain: time course

Project description:We observed the expression profile of the total mRNA of TTHB212-deficient of Thermus thermophilus HB8 strain grown in a rich medium at 70°C for 7 and 8 hours

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T. thermophilus HB8 wild-type strain in response to iron (2008)

Project description:We observed the expression profile of the total mRNA in Thermus thermophilus HB8 wild-type strain at 30 min after the addition of FeSO4.

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SuperSeries for the analysis of transcriptional regulation by Thermus themophilus CsoR (TTHA1719)

Project description:We observed the copper response of Thermus thermophilus HB8 wild-type and csoR deficient strain at 0 and 30 min after the addition of 1.25 mM CuSO4, and zinc response of T. thermophilus HB8 wild-type strain at 0 and 30 min after the addition of 1.0 mM ZnSO4. This SuperSeries is composed of the SubSeries listed below.

2010-04-13 | GSE19521 | GEO

Thermus thermophilus HB8

Project description:Thermus thermophilus HB8

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Differential expression analysis of T. thermophilus HB8 TTHA0995-deficient strain in response to iron

Project description:We observed the expression profile of the total mRNA in Thermus thermophilus HB8 TTHA0995 deficient strain at 0 and 30 min after the addition of FeSO4.

2010-10-15 | GSE21470 | GEO

Differential expression analysis of T. thermophilus HB8 wild-type strain in response to iron

Project description:We observed the expression profile of the total mRNA in Thermus thermophilus HB8 wild-type strain at 0 and 30 min after the addition of FeSO4.

2010-10-15 | GSE21199 | GEO

Differential expression analysis of T. thermophilus HB8 csoR deficient strain in response to copper.

Project description:We observed the expression profile of the total mRNA in Thermus thermophilus HB8 csrR deficient strain at 0 and 30 min after the addition of CuSO4.

2010-04-13 | GSE19509 | GEO

Differential expression analysis of T. thermophilus HB8 wild-type strain in response to copper.

Project description:We observed the expression profile of the total mRNA in Thermus thermophilus HB8 wild-type strain at 0 and 30 min after the addition of CuSO4.

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