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MiRNA profiling of sacral chordoma and nucleus pulposus cells

ABSTRACT: Next-generation RNA sequencing (NGS) was used for the combinatorial analysis of mRNA-miRNA gene expression profiles in sacral chordoma and nucleus pulposus samples, and to predict miRNA-mRNA regulatory networks with altered activity in chordoma. We identified a gene set including key regulators of the Hippo pathway, which is targeted by differently expressed miRNAs, and validated their altered expression by RT-qPCR. These newly identified miRNA/RNA interactions are predicted to have a role in the self-renewal process of chordoma stem cells, which might sustain the high rate of recurrence for this tumor.

ORGANISM(S): Homo sapiens

PROVIDER: GSE209695 | GEO | 2022/07/28

REPOSITORIES: GEO

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