Single-nucleus total RNA-sequencing of C2C12 myoblasts via in situ polyadenylation

ABSTRACT: Current methods for single-nucleus RNA-sequencing (snRNAseq) are limited to assaying endogenously polyadenylated (A-tailed) RNA transcripts. Here we demonstrate that enzymatic in situ polyadenylation of RNAs enables detection of the full spectrum of RNAs, expanding the scope of sequencing-based single-nucleus transcriptomics to the total transcriptome. We apply standard snRNAseq and total snRNAseq to C2C12 myoblasts. Our in situ polyadenylation strategy relies on a brief, low-cost add-on to a widely used protocol for single-cell/single-nucleus RNA-sequencing, and thus could be broadly and quickly adopted. Single-nucleus RNA-sequencing of the total transcriptome will enable new insights into gene regulation and biology.

ORGANISM(S): Mus musculus

PROVIDER: GSE209780 | GEO | 2022/08/01

REPOSITORIES: GEO