Transcriptomics

Dataset Information

46

Expression data from different stages and types of oocytes and cumulus cells (CCs)


ABSTRACT: The bidirectional communication between bovine oocytes and CCs is vital for functioning and development of both cell types. We used microarray to identify genes which are differentially expressed between germinal vesicle (GV)- and metaphase II (MII)-stage oocytes and CCs and those differentially expressed when oocytes mature with or without the other. We also identified genes differentially expressed between CCs at GV and MII stages. Overall design: Slaughterhouse ovaries were collected and GV-stage cumulus oocyte complexes (COCs) were aspirated. Different stages and types of oocytes and CCs were used for total RNA isolation and hybridisation on Affymetrix microarray.

INSTRUMENT(S): [Bovine] Affymetrix Bovine Genome Array

ORGANISM(S): Bos taurus  

SUBMITTER: Alemu Regassa 

PROVIDER: GSE21005 | GEO | 2010-03-23

SECONDARY ACCESSION(S): PRJNA125787

REPOSITORIES: GEO

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