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Transcriptome analysis of the binucleate ciliate Tetrahymena thermophila cells after mating and re-feeding.

ABSTRACT: During macronuclear development the five germline chromosomes undergo massive rearrangement, and the entire epigenome is remodeled from an obligate heterochromatic to obligate euchromatic state. The ~180 unique macronuclear chromosomes undergo two development waves of endo-reduplication to achieve a final copy number of ~45C, while the 21 kb rDNA minichromosome is amplified to ~9000C. The first period (endoreplication phase I) involves two rounds of DNA replication and generates an 8 C macronucleus (6–18 h after mating). Upon re-feeding, a second re-replication period (endoreplication phase II) is initiated generating a macronuclear DNA content of 32–64 C. This study focused on RNA expression profile of cell in endoreplication phase II.

ORGANISM(S): Tetrahymena thermophila

PROVIDER: GSE210103 | GEO | 2025/07/29

REPOSITORIES: GEO

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Mating of the stichotrichous ciliate Oxytricha trifallax induces production of a class of 27nt small RNAs derived from the parental macronucleus

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Mating of the stichotrichous ciliate Oxytricha trifallax induces production of a class of 27nt small RNAs derived from the parental macronucleus

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Transcriptome analysis of the binucleate ciliate Tetrahymena thermophila with asynchronous nuclear cell cycles

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