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High-Throughput Isolation of RNA from Single Cells within Intact Tissue for Spatial and Temporal Sequencing

ABSTRACT: Obtaining and performing single-cell RNAseq by aspirating only1-5% of sub-single cell RNA content from individual cells within fresh frozen tissue samples.

ORGANISM(S): Homo sapiens

PROVIDER: GSE210115 | GEO | 2022/09/30

REPOSITORIES: GEO

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Glia-specific nuclei isolation and single cell transcriptomics inform astrocyte pathology in human temporal lobe epilepsy

Project description:The role of human glia in many neurological disorders is still poorly understood due to the lack of tools that reliably isolate specific glial subpopulations from bulk tissue, directly from their native niche. To better understand the contributions of glial pathology in human epilepsy, we developed and validated a novel sorting strategy that simultaneously isolates nuclei populations of astrocytes (PAX6+), oligodendroglial progenitors (OPCs) (OLIG2+) and neurons (NEUN+) from non-pathological fresh-frozen human postmortem temporal neocortex brain tissue (TL Control) and then employed it, in combination with single cell RNA-seq, to characterize the cell-type specific transcriptome alterations in epilepsy temporal neocortex derived from fresh surgical material in patients with medically refractory temporal lobe epilepsy (TLE).

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