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Gene expression analysis of HepaRG cells that ectopically express miR-371-3 cluster

ABSTRACT: the immoratlized hepatic cell line HepaRG was transduced with a retroviral vector containing the miR-371-3 cluster. As a control, transduction was performed with the empty vector. Transcriptome analysis was performed by using the Affymetrix whole-transcript human gene 1.0 ST array. Each condition was analyzed in triplicate

ORGANISM(S): Homo sapiens

PROVIDER: GSE21080 | GEO | 2012/12/31

SECONDARY ACCESSION(S): PRJNA129679

REPOSITORIES: GEO

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Gene expression analysis of HepaRG cells that ectopically express miR-371-3 cluster

Project description:the immoratlized hepatic cell line HepaRG was transduced with a retroviral vector containing the miR-371-3 cluster. As a control, transduction was performed with the empty vector. Transcriptome analysis was performed by using the Affymetrix whole-transcript human gene 1.0 ST array. Each condition was analyzed in triplicate hierarchical unsupervised clustering of HB samples according to their miRNA expression profile identifies 2 tumor subgroups associated with different degree of differentiation, proliferation rate and invasive phenotype. As subset of these tumors is used as training set to build a mir classifier. When the signature is applied to an independent test set, an independent series of HB is classified patients into two groups associated with the clinical features identified in the first set of patients.

2012-12-31 | E-GEOD-21080 | biostudies-arrayexpress

Analysis of Hepatoblastoma and normal fetal liver miRNA profiles and HepaRG cells that ectopically express miR-371-3 cluster

Project description:This SuperSeries is composed of the SubSeries listed below.

2012-12-13 | GSE21085 | GEO

Analysis of Hepatoblastoma and normal fetal liver miRNA profiles and HepaRG cells that ectopically express miR-371-3 cluster

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2012-12-13 | E-GEOD-21085 | biostudies-arrayexpress

The Co-regulated Genes by Ectopic Expression of MiR-371-372-373 Cluster in HepG2 Cells

Project description:MicroRNAs are important cellular regulators and their dysfunctions are associated with various disease. miR-371/372/373 was found co-regulated in HBV-producing HepG2.2.15 cells when compared to its non-HBV producing maternal HepG2 cells. To obtain a glimpse of the potential influence of the enforced miR-371-372-373 cluster in HepG2 gene expression, a two-color Capitalbio 70-mer oligo microarray platform, which contained 21,329 well-characterized human gene probes, was used to identify the differentially expressed genes between miR-371-372-373-HepG2 and mock-HepG2 in two independent biological replicate.

2012-01-15 | GSE19981 | GEO

The Co-regulated Genes by Ectopic Expression of MiR-371-372-373 Cluster in HepG2 Cells

2012-01-15 | E-GEOD-19981 | biostudies-arrayexpress

Identification of differentially expressed genes between control and miR-371~373 cluster-overexpressing colorectal cancer cells

Project description:The miR-371~373 cluster is a suspected repressor of colon cancer initiation and progression. To better understand its role in metastasis initiation, we used microarray expression analysis to identify potential target genes of this miRNA cluster Potential target genes were defined as significantly downregulated genes after stable overexpression of the miR-371~373 cluster

2018-07-23 | GSE102001 | GEO

Coemansia sp. RSA 371 isolate:RSA 371

Project description:Coemansia sp. RSA 371 Resequencing

| PRJNA712424 | ENA

Functional screening implicates miR-371-3p and peroxiredoxin 6 in reversible tolerance to cancer drugs [SPR1108]

Project description:Acquired resistance to cancer drug therapies almost always occurs in advanced-stage patients even following a significant response to treatment. In addition to mutational mechanisms, various non-mutational resistance mechanisms have now been recognized. We previously described a chromatin-mediated subpopulation of reversibly drug-tolerant persisters (DTPs) that is dynamically maintained within a wide variety of tumor cell populations. Here, we explored a potential role for microRNAs in such transient drug tolerance. Functional screening of 879 human microRNAs revealed miR-371-3p as a potent suppressor of drug tolerance. PRDX6 (peroxiredoxin 6) was identified as a key target of miR-371-3p in establishing drug tolerance by regulating PLA2/PKCÎ± activity and reactive oxygen species. PRDX6 expression is associated with poor prognosis in cancers of multiple tissue origins. These findings implicate miR-371-3p as a suppressor of PRDX6 and suggest that co-targeting of PRDX6 or modulating miR-371-3p expression together with targeted cancer therapies may delay or prevent acquired drug resistance. PC9 stable cell lines expressing GFP vector or miR-371-3p were generated to study the effect of miR-371-3p in response to erlotinib treatment for RNA extraction and hybridization on Affymetrix microarrays. Stable cell lines were treated with DMSO or erlotinib for 24hrs and RNA isolated for analyzing the effect of miR-371-3p on gene expression upon erlotinib treatment.

2016-08-01 | E-GEOD-83120 | biostudies-arrayexpress

The eutherian-specific miR-290/miR-371 cluster modulates placental growth and maternal-fetal transport

Project description:A family of vertebrate-specific microRNAs called the ESCC microRNAs regulates proliferation and differentiation of embryonic stem cells. The ESCC microRNAs arise from two genetic loci in mammals, the miR-290/miR-371 and miR-302 loci. While the miR-302 locus is found broadly among vertebrates, the miR-290/miR-371 locus is unique to eutherian species, suggesting a role in placental development. Here, we evaluate that role. A knockin reporter for the mouse miR-290 gene is expressed throughout the embryo until gastrulation, at which time it becomes specifically expressed in extraembryonic tissues and the germline. In the placenta, expression is limited to the trophoblast lineage, where it remains highly expressed until birth. Deletion of the miR-290 gene results in reduced trophoblast progenitor cell proliferation and a reduced DNA content in endoreduplicating trophoblast giant cells. A reduction in placental size precedes a reduction in fetal size and prenatal death of most knockout embryos. The vascular labyrinth shows disorganization with thickening of the barrier between maternal and fetal blood associated with reduced diffusion of a radioactive tracer. Multiple mRNA targets of the cluster miRNAs are upregulated. Together, these data uncover a critical function for the miR-290 in the regulation of a network of genes required for normal placental development, suggesting a central role for this microRNA cluster in the evolution of eutherian species.

2017-03-04 | GSE95687 | GEO

Hepatocytes-HepaRG reprogramming.

Project description:To identify the molecular mechanisms and environmental inducers contributing to reprogramming of hepatocytes into progenitors in HCC context, we used the HepaRG cell line as model.

2013-12-05 | GSE52989 | GEO

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