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Single cell RNA sequencing of macrophage-depleted mammary gland by clodronate liposome treatment

ABSTRACT: To investigate the impact of macrophages on cell population of mammary gland, we performed the single cell RNA sequencing of the macrophage-depleted mammary gland compared with control.

ORGANISM(S): Mus musculus

PROVIDER: GSE210825 | GEO | 2023/04/20

REPOSITORIES: GEO

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Mammary gland

Project description:Goat Mammary gland RNA-Seq (DGE)

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Resident macrophages promote homeostasis of hyaluronan-associated extracellular matrix in the mammary gland stroma

Project description:Tissue resident macrophages in the mammary gland are found in close association with epithelial structures and within the adipose stroma, and are important for mammary gland development and tissue homeostasis. While epithelial-associated macrophages have been linked to ductal development, the contributions of stromal macrophages to mammary gland homeostasis remain unknown. Using transcriptional profiling, we identify a distinct resident stromal macrophage subpopulation that is characterized by expression of Lyve-1, a receptor for the extracellular matrix component hyaluronan. This subpopulation is enriched in genes associated with extracellular matrix remodeling and is found to be specifically associated with hyaluronan-rich regions within the mammary stroma. Furthermore, macrophage depletion leads to increased accumulation of hyaluronan-associated extracellular matrix in the mammary stroma. These results demonstrate the presence of a distinct subpopulation of macrophages and provide insights into the functional contributions of these macrophages to stromal homeostasis in the mammary gland.

2020-05-31 | GSE148207 | GEO

Gene expression of myeloid lineage cells reveals a role for myeloid progenitor cells in mammary gland fibrosis in obesity

Project description:Obesity enhances macrophage recruitment in to mammary adipose tissue and increases fibrosis. We isolated monocytes/macrophages and myeloid progenitor cell populations from the mammary gland and queried gene expression of fibrosis related genes to assess the contribution of each population to fibrosis within the mammary gland.

2023-08-01 | GSE225841 | GEO

Exosomal protein identification from untreated mice and macrophage-depleted mice

Project description:Exosomes are important mediators for cell-cell communication. In previous study, the clearance of exosomes were markedly delayed in systemic circulation of macrophage-depleted mice. To reveal the possible molecular regulation involved in exosome clearance, we performed exosomal proteomic analysis on MP-Exo from macrophage depleted mice and control ones.

2020-02-27 | PXD012885 | JPOST Repository

M/D-tumors vs non´-tumor mammary gland tissues

Project description:The main goal of this experiment was to contrast the gene expression of mammary gland tissues at three different tumoral stages : M/D-driven mammary gland small tumors vs mammary gland tissues that have been exposed to M/D but they did not develop a tumor (hyperplastic mammary gland) vs mammary gland tissues that were NOT expossed to M/D (normal mammary gland). Expression profile of 18 mice mammary gland tissues at 3 differents neoplastic stages before and after M/D expossure

2020-06-22 | GSE151197 | GEO

RNA-seq of goat mammary gland cells samples from different treatment groups

Project description:The goal of this project is to explore lncRNA55666 regulation goat mammary gland lipid metabolism. We tried to search the mechanism of lncRNA55666 regulation lipid metabolism. RNA-seq of goat mamamary gland cells samples from different groups: 5NC, lncRNA55666 overexpression, 3NC, lncRNA55666 knockdown. The goat mammary gland cells were cultured in 3D condition. The cell were transfected with virus with lncRNA55666 gene (overexpression), or inhibition of lncRNA expression (lncRNA gene knockdown).

2020-08-01 | GSE135692 | GEO

Mammary gland tumor immunoinfiltrate analysis

Project description:Mammary gland tumor immunoinfiltrate analysis

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Mammary gland tumor immunoinfiltrate analysis

Project description:Mammary gland tumor immunoinfiltrate analysis

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Distinct lactation-associated macrophages exist in murine mammary tissue and human milk

Project description:Macrophages are involved in immune defense, organogenesis and tissue homeostasis. They also contribute to the different phases of mammary gland remodeling during development, pregnancy and involution post-lactation. Yet, less is known about the dynamics of mammary gland macrophages in the lactation stage. Here, we describe a macrophage population present during lactation in mice. By multi-parameter flow cytometry and single-cell RNA sequencing we reveal this population as distinct from the two resident macrophage subsets present pregestationally. These lactation-induced macrophages (LiMacs) are predominantly monocyte-derived and expand by proliferation in situ concomitant with nursing. LiMacs develop independently of IL-34 but require CSF-1 signaling and are partly microbiota-dependent. Locally, they reside adjacent to the basal cells of the alveoli and extravasate into the milk. Moreover, we also found several macrophage subsets in human milk, resembling LiMacs. Collectively, these findings reveal the emergence of unique macrophages in the mammary gland and milk during lactation.

2023-06-23 | PXD041711 | Pride

polyA depleted RNA-seq from mammary epithelial cell (ENCSR000CPU)

Project description:The libraries contained in this experiment come from mammary gland epithelial primary whole cells, HMEC isolated from independent donors. They are stranded PE76 Illumina GAIIx RNA-Seq libraries from rRNA-depleted and DNS normalized Poly-A- RNA > 200 nucleotides in size. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

2016-03-18 | GSE78596 | GEO

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