Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene Expression Profiling of the Calvaria from Conditional Hdac3 Defecient and Wild-type Mice

ABSTRACT: We utilized the Illumina MouseRef-8 gene expression technology to quantify differential gene expression between wildtype mice and mice with Osterix driven Cre conditional knockout of Hdac3 (Hdac3-CKO). We compared the RNA extracted from calvaria from 8 wildtype and 8 conditional knockout litter matched mice using two separate Illumina MouseRef-8 chips.

ORGANISM(S): Mus musculus

PROVIDER: GSE21087 | GEO | 2010/03/27

SECONDARY ACCESSION(S): PRJNA126703

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Gene Expression Profiling of the Calvaria from Conditional Hdac3 Defecient and Wild-type Mice

Project description:We utilized the Illumina MouseRef-8 gene expression technology to quantify differential gene expression between wildtype mice and mice with Osterix driven Cre conditional knockout of Hdac3 (Hdac3-CKO). We compared the RNA extracted from calvaria from 8 wildtype and 8 conditional knockout litter matched mice using two separate Illumina MouseRef-8 chips. Mice with exon 7 of Hdac3 flanked by loxP sites were crossed with mice expressing Cre driven by the Osterix promoter. RNA from 5 day old mouse calvarial explants (digested for 20 minutes with collagenase) was purified using TRIzol according to the manufacturerâs protocol (Invitrogen) and reverse transcribed using Qiagenâs Quantitect Reverse Transcription Kit. Two independent microarray experiments were performed; each experiment used RNA from four wildtype and four conditional knockout litter matched mouse calvaria.

2010-04-07 | E-GEOD-21087 | biostudies-arrayexpress

Microarray of RNA from calvaria in WT and OASIS-/- (KO) mice

Project description:Investigation of whole genome gene expression level changes in OASIS KO calvaria compared to wild-type calvaria. To gain further insight into the potential mechanisms underlying the defective bone formation in OASIS KO mice, we compared the gene expression in calvaria between WT and OASIS KO mice using a microarray.

2009-09-10 | GSE18062 | GEO

Differentiation Time Course Analysis of Gene Expression in Primary Osteoblasts from Wild Type and β-Arrestin 2 Knockout Mice

Project description:Gene expression analysis was performed on differentiating obsteoblasts isolated from calvaria of C57BL/6J wildtype or B-arrestin 2 knockout mice.

2022-08-16 | GSE181459 | GEO

Quantitative Analysis of mRNA expression in calvaria tissue from control and Med23-/- vs. wild type and Runx2+/- mice through Next Generation Sequencing

Project description:We sequenced mRNA from 12 single newborn mouse calvaria tissues ( from 3 control and 3 Med23-/- cKO mice from a litter; 3 wildtype and 3 Runx2+/- mice from another litter) to investigate the correlation between Med23 and Runx2 in terms of affect on the mRNA level by their deficiencies. We find there is a positiove correlation in gobal gene expression affected by defiencies of the two genes.

2016-01-21 | GSE77007 | GEO

Microarray of RNA from calvaria in WT and OASIS-/- (KO) mice

2010-05-05 | E-GEOD-18062 | biostudies-arrayexpress

Salvi_PNAS_Phosphoproteome_ATRwt-cko

Project description:Phosphoproteomic data obtained from mouse muscle stem cells from wildtype and muscle stem cell specific conditional ATR knockout mice

2022-04-01 | PXD028432 | Pride

Mus musculus

Project description:Gene Expression Profiling of the Calvaria from Conditional Hdac3 Defecient and Wild-type Mice

| PRJNA126703 | ENA

Axin2/Runx2 mouse calvaria expression

Project description:Axin2/Runx2 mouse calvaria expression

| PRJNA254341 | ENA

Multi-omics and 3D-imaging reveal bone heterogeneity and unique calvaria cells in neuroinflammation

Project description:Diverse immune cells move from the calvaria marrow into the dura mater via recently discovered skull-meninges connections (SMCs). Yet, how the calvaria bone marrow is different from the other bones and whether it plays a special role in human diseases remain unknown. Using multi-omics approaches and whole mouse transparency we reveal that bone marrow cells are highly heterogeneous across the mouse body. The calvaria harbors the most distinct molecular signature with hundreds of differentially expressed genes and proteins. Acute brain injury induces skull-specific outcomes including increased calvaria cell numbers. Moreover, TSPO-PET imaging of stroke, dementia and multiple sclerosis patients demonstrate increased inflammation in the human skull, mirroring the underlying brain inflammation.

2024-01-17 | PXD030250 | Pride

Detection of putative PKA target phosphorylation sites in Atg5flox:CamKII-Cre wildtype and knockout mice brain tissue

Project description:The experiment was performed to identify PKA phosphorylation substrates in wildtype and autophagy-deficient brains. Therefore, hippocampus and cortex of wildtype and conditional knockout mice were isolated and sliced. The brain slices were incubated with DMSO or Forskolin, a cAMP elevating agent, to induce PKA activity. Samples were measured by LC-MS/MS and were used to quantify proteomic and phosphoproteomic changes.

2022-11-15 | PXD031106 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data