Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA binding profile of MED23 in 293T cells

ABSTRACT: To delineate the repertoire of RNA binding of MED23, we performed a denatured CLIP-seq experiment coupled with high-salt wash in MED23-HA 293T cells and wildtype 293T cells as a control.

ORGANISM(S): Homo sapiens

PROVIDER: GSE211081 | GEO | 2025/05/28

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Genomic occupation profile of Mediator MED23 in 293T cells

Project description:We performed CUT&Tag experiments in MED23-HA and WT 293T cells to analyze the genomic occupation profiles for the Mediator MED23.

2025-05-28 | GSE211082 | GEO

Transcription profiles of MED23-KD and wildtype 293T cells

Project description:To characterize the function of MED23 on transcription, we generated two MED23-KD 293T cell lines in which MED23 was about 75% knockouted by CRISPR-Cas9. Then we performed RNA-seq to analyze the change of transcript levels around termination sites.

2025-05-28 | GSE211083 | GEO

RNA polymerase II binding profile in WT and MED23-KD 293T cells

Project description:MED23-KD 293T cells in which MED23 was mostly knockouted were generated by CRISPR-Cas9. We performed CUT&Tag experiments in WT and MED23-KD cells to characterize the change of Pol II binding.

2025-05-28 | GSE211084 | GEO

The occupied profile of FIP1 in WT and MED23-KD 293T cells

Project description:MED23-KD 293T cells in which MED23 was mostly knockouted were generated by CRISPR-Cas9. We performed ChIP experiments in WT and MED23-KD cells to profile the genomic occupation profiles of FIP1, which is one of the components of pre-mRNA 3' end processing complex.

2025-05-28 | GSE211080 | GEO

RNA binding profile of MED23 in 293T cells

Project description:RNA binding profile of MED23 in 293T cells

| PRJNA868786 | ENA

Nascent RNA profiles of WT and MED23-KD 293T cells

Project description:To profile the nascent RNAs, we performed 4SU-seq. Briefly, WT or MED23-KD 293T cells were seeded at 50% confluence in a 10 cm dish and cultured overnight. The newly transcribed RNAs in living cells were then transiently labelled with 4SU at a final concentration of 1 mM for 15 minutes. The labelled cells were harvested immediately with TRIzol. Total RNA was extracted with phenol/chloroform/isoamyl alcohol. The 4SU-labelled RNAs were biotinylated and enriched using streptavidin beads. Finally, the enriched nascent RNAs were subjected to library construction using a NEBNext Ultra Directional RNA Library Prep Kit for Illumina for sequencing.

2025-05-28 | GSE282498 | GEO

Transcription profiles of MED23-KD and wildtype 293T cells

Project description:Transcription profiles of MED23-KD and wildtype 293T cells

| PRJNA868784 | ENA

Genomic occupation profile of Mediator MED23 in 293T cells

Project description:Genomic occupation profile of Mediator MED23 in 293T cells

| PRJNA868785 | ENA

Nascent RNA profiles of WT and MED23-KD 293T cells

Project description:Nascent RNA profiles of WT and MED23-KD 293T cells

| PRJNA1189027 | ENA

RNA polymerase II binding profile in WT and MED23-KD 293T cells

Project description:RNA polymerase II binding profile in WT and MED23-KD 293T cells