Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA sequencing of LSKs in the bone marrow from normal and 5-FU treated WT and Tespa1-null mice

ABSTRACT: LSKs(lineage-, Sca-1+, c-kit+ cells) in the bone marrow from normal and 5-Fluorouracil (5-FU) treated (Day 9) wild type (WT) and Tespa1-knockout (KO) mice were isolated to explore the differentially expressed genes by RNA-seq.

ORGANISM(S): Mus musculus

PROVIDER: GSE211292 | GEO | 2023/05/17

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

RNA sequencing of LSKs in the bone marrow from WT and MC5R-null mice

Project description:LSKs(lineage-, Sca-1+, c-kit+ cells) in bone marrow from wild type (WT) and MC5R-knockout (KO) mice after after irradiation were isolated to explore the differentially expressed genes by RNA-seq.

2023-03-15 | GSE210204 | GEO

RNA sequencing of LSKs in the bone marrow from irradiated WT and Tespa1-null mice

Project description:LSKs (lineage-, Sca-1+, c-kit+ cells) in the bone marrow from wild type (WT) and Tespa1-knockout (KO) mice at day 13 after 5.0 Gy irradiation were isolated to explore the differentially expressed genes by RNA-seq.

2023-05-17 | GSE211117 | GEO

HSC 5-FU time course

Project description:HSC (Sca+ SP) were isolated from 8-12 week C57B6 mice at various time points after treatment with 5-Fluorouracil. RNA was isolated from 50,000-100,000 FACS sorted cells and subjected to two rounds of T7 based linear amplification using Ambion's Message Amp kit. Two replicates from each time point were analyzed. http://www.plosbiology.org/plosonline/?request=get-document&doi=10.1371%2Fjournal.pbio.0020301 Keywords = HSC Keywords = proliferation Keywords = quiescence Keywords = 5-FU Keywords: time-course

2004-09-28 | GSE1559 | GEO

ATAC-seq of hematopoietic stem cells in bone marrow after 5-FU administration

Project description:Purpose: The goals of this study are to elucidate the underlying mechanism for the regulation of HSC divisions. Methods: Before or after wild type (WT) mice were treated with 5-fluorouracil (5-FU), 15000 cells of HSCs were sorted. These cells were subjected to ATAC-seq using Next-seq. The sequence reads that passed quality filters were analyzed by FASTX-toolkit.

2022-01-19 | GSE164281 | GEO

CUT&Tag-seq for H3K27ac in hematopoietic stem cells in bone marrow after 5-FU administration

Project description:Purpose: The goals of this study are to elucidate the underlying mechanism for the regulation of HSC divisions. Methods: Before or after wild type (WT) mice were treated with 5-fluorouracil (5-FU), 12500 cells of HSCs were sorted. These cells were subjected to CUT&Tag-seq to analyze H3K27ac profile by using Next-seq. The sequence reads that passed quality filters were analyzed by Trimmomatic.

2022-01-19 | GSE190148 | GEO

mRNA profiles of hematopoietic stem or progenitor cells in bone marrow after 5-FU administration

Project description:Purpose: The goals of this study are to elucidate the underlying mechanism for the regulation of HSC divisions. Methods: Before or after wild type (WT) mice were treated with 5-fluorouracil (5-FU), 100 cells of HSCs were sorted. Moreover, after HSCs were cultured for 1day, 100 cells of phenotypic HSCs were sorted. These cells were subjected to mRNA sequence using Next-seq. The sequence reads that passed quality filters were analyzed by CLC genomic workbench.

2022-01-19 | GSE164283 | GEO

mRNA profiles of bone marrow and splenic hematopoietic stem cells after 5-FU challenge

Project description:Under stress hematopoiesis, previous studies have suggested the migration of hematopoietic stem cells (HSCs) from bone marrow (BM) to extramedullary sites such as spleen. However, there is little direct evidence of HSC migration from BM to spleen. Here, we induced myeloablation via 5-fluorouracil (5-FU) and showed the direct evidence of HSC migration from BM to spleen during hematopoietic regeneration via a photoconvertible fluorophore. We found that during hematopoietic regeneration, there were mechanistic differences for HSC migration during early (day 3 to 8) and late phase (day 11 to 14). During steady-state, HSCs preferentially migrated to BM rather than spleen, but during the early phase HSC migration to spleen predominated. Indeed, in the early phase, HSC mobilization from BM was induced in G-CSF-dependent manners, while HSCs in the late phase gained significantly enhanced cell-autonomous motility, which was independent of chemotaxis. Collectively, HSC migration was dynamically changed during hematopoietic regeneration.

2023-12-01 | GSE248234 | GEO

Analysis of gene expression profile in 5-fluorouracil (5-FU)-resistant human colon cancer cells

Project description:We analyzed the differentially regulated genes in 5-fluorouracil-resnstant human colon cancer cells to discover novel biomarkers involved in 5-FU resistance in colorectal cancer.

2017-03-27 | GSE56322 | GEO

Gene expression profile at single cell level of Lineage- Sca1+ c-Kit+ cells (LSKs) from the bone marrow of normal C57BL/6J mice with or without 5.0 Gy total body irradiation.

Project description:Lineage- Sca1+ c-Kit+ cells (LSKs) are considered as a homogeneous population in the bone marrow. We used single cell RNA sequencing (scRNA-seq) to analyze the diversity of LSKs in the bone marrow.

2023-04-22 | GSE221907 | GEO

miRNA profiling data of mouse bone marrow samples four days post 5-FU treatment versus untreated controls

Project description:To identify miRNAs that regulate hematopoietic response after 5-FU-induced injury, we analyzed miRNA expression changes in bone marrow cells from mice with or without 5-FU treatment. Global miRNA expression profiling of the 5-FU and untreated cohorts showed that 92 miRNAs (among 168 that passed the detection threshold) were significantly upregulated or downregulated, with false discovery rates < 5%.

2012-09-24 | GSE39211 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data