Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Functional dissection of human cardiac enhancers with hiPSC-CMs and lentiMPRA

ABSTRACT: To assess human cardiac enhancer activity, 400 bp candidate cardiac enhancers and controls were tested in iPSC-CMs using lentiMPRA.

ORGANISM(S): Homo sapiens

PROVIDER: GSE211417 | GEO | 2023/11/16

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

RNA-seq in healthy and SQT1 hiPSC-CMs upon manipulation of miR-365

Project description:Abnormalities of ventricular action potential (AP) cause malignant cardiac arrhythmias and sudden cardiac death. Here, we sought to identify microRNAs (miRNAs) that regulate the human cardiac AP and asked whether their manipulation allows for therapeutic modulation of AP abnormalities. Quantitative analysis of the miRNA targetomes in human cardiac myocytes identified miR-365 as a primary miRNA to regulate repolarizing ion channels. AP recordings in patient-specific induced pluripotent stem cell-derived cardiac myocytes (iPSC-CMs) showed that elevation of miR-365 level significantly prolonged AP duration in hiPSC-CMs derived from a Short-QT syndrome (SQTS) patient, whereas specific inhibition of miR-365 normalized pathologically prolonged AP in Long-QT syndrome (LQTS) iPSC-CMs. Transcriptome analyses in human iPSC-CMs at bulk and single-cell level corroborated the key cardiac repolarizing channels as direct targets of miR-365, together with functionally synergistic regulation of additional AP-regulating genes by this miRNA. Whole-cell patch clamp experiments revealed miR-365-based regulation of repolarizing ionic currents. Finally, refractory period measurements in human myocardial slices substantiated the prolonging effect of miR-365 on AP duration also in adult human myocardial tissue. Our results delineate miR-365 to regulate human cardiac AP duration by targeting key factors of cardiac repolarization.

2021-12-01 | GSE185687 | GEO

single cell RNA-seq in healthy and LQT1 hiPSC-CMs upon manipulation of miR-365

2021-12-01 | GSE185688 | GEO

Functional analysis of human cardiac enhancers by tiling mutagenesis

Project description:To further determine the sequence features of active cardiac enhancers, 123 cardiac enhancers were functionally dissected by 10 bp tiling deletion-based mutagenesis MPRA in iPSC-CMs.

2023-11-16 | GSE208284 | GEO

Transcriptomic analysis of the effect of iron overload in human iPSC-CMs

Project description:We used human iPSC-CMs generated from healthy individuals and performed RNA-sequencing after 5 days of trastuzumab treatment to examine the mechanism associated with cardiac dysfunction in iPSC-CMs after iron treatment. Transcriptome analysis revealed broad changes in cardiovascular development and processes.

2020-06-08 | GSE141678 | GEO

Analysis of CHD non-coding DNVs for their impact on cardiac cis-regulatory element activity

Project description:6761 CHD non-coding DNVs were prioritized from 749 unexplained CHD trios and functionally assessed in iPSC-CMs using lentiMPRA.

2023-11-16 | GSE208285 | GEO

Effect of long-term ethanol treatment in human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs)

Project description:We treated our iPSC-CMs with EtOH for 30+ days to investigate changes in gene expression

2023-03-16 | GSE218604 | GEO

Transcriptome analysis of hiPSC-CMs carrying FLNC mutations

Project description:In this study we performed transcriptome analysis of cardiomyocytes (CMs) derived from two hiPSC lines of patients with V2264M and R1267Q mutations in FLNC gene. hiPSC cell line were obtained from patient’s PBMCs with FLNC mutation using CytoTune-iPS 2.0 Sendai Reprogramming Kit (Thermo Fisher Scientific). Detailed information about iPSC line characterization can be found in hiPSCreg database: (FAMRCi011-A and FAMRCi009). Information about FAMRCi009 is earlier published DOI: 10.1016/j.scr.2021.102640.

2024-03-05 | GSE260653 | GEO

Rhesus iPSC-CMs response to anoxia, comparing with human iPSC-CMs

Project description:Rhesus iPSC-CMs response to anoxia, comparing with human iPSC-CMs

| PRJNA428243 | ENA

Rhesus iPSC-CMs response to anoxia, comparing with human iPSC-CMs

Project description:RNA-Seq to compare hypoxia responses between human and rhesus macaque iPSC-CMs

2018-09-10 | GSE108676 | GEO

The role of MEF2A in iPSC-CMs maturation

Project description:To understand the role of MEF2A in iPSC-CMs maturation, we used MEF2A-siRNA to reduce MEF2A transcription in iPSC-CMs and then examined the changes in transcription levels

2023-02-03 | GSE196655 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data