Dataset Information

Desciption of the transcriptome profiles of mouse heart innervating DRG and Nodose Ganglia-derived sensory neurons

ABSTRACT: To obtain the mRNA expression profiles of vagal and spinal cardiac sensory neurons, mouse Dorsal Root Ganglia (DRG) and Nodose Ganglia (NG) neurons innervating the heart were labelled by retrograde Di-8-ANEPPQ dye which was injected to the heart. Then bilateral NGs and DRGs between C-T segments were dissected and Di-8-ANEPPQ-labelled DRG and NG neurons were FACS sorted. Finally, Di-8-ANEPPQ+ DRG and NG neurons were subjected to total RNA extraction and library preparation protocols for RNA-seq experiment. For control samples, total NG and DRG (C-T) neurons were dissected from the wt mice and same RNA extraction and library preparation protocols were applied. Finally, mRNA profiles of heart-spesific DRG and NG neurons were compared to those of total DRG and NG populations, respectively.

ORGANISM(S): Mus musculus

PROVIDER: GSE211622 | GEO | 2025/08/19

REPOSITORIES: GEO

ACCESS DATA

Dataset's files

Source:

			Action	DRS
		Other
	GSE211622_fpkm_values_matrix.txt.gz	Txt

Items per page:

1 - 2 of 2

Similar Datasets

Project description:Vagal afferent neurons are thought to convey primarily physiological information, whereas spinal afferents transmit noxious signals from the viscera to the central nervous system. In order to elucidate molecular identities for these different properties, we compared gene expression profiles of neurons located in nodose ganglia (NG) and dorsal root ganglia (DRG) in mice. Intraperitoneal administration of Alexa Fluor-488 conjugated Cholera toxin B allowed identification of neurons projecting to the viscera. Fluorescent neurons in DRG (from T10 to T13) and NG were isolated using laser capture microdissection. Gene expression profiles of visceral afferent neurons, obtained by microarray hybridization, were analysed using multivariate spectral map analysis, SAM algorithm (Significance Analysis of Microarray data) and fold-difference filtering. A total of 1996 genes were found to be differentially expressed in DRG versus NG, including 41 G-protein coupled receptors and 60 ion channels. Expression profiles obtained on laser-captured neurons were contrasted to those obtained on whole ganglia demonstrating striking differences and the need for microdissection when studying visceral sensory neurons because of dilution of the signal by somatic sensory neurons. Furthermore, a detailed catalogue of all adrenergic and cholinergic, GABA, glutamate, serotonin and dopamine receptors, voltage-gated potassium, sodium and calcium channels and transient receptor potential cation channels present in visceral afferents is provided. Our genome-wide expression profiling data provide novel insight into molecular signatures that underlie both functional differences and similarities between NG and DRG visceral sensory neurons. Moreover, these findings will offer novel insight into mode of action of pharmacologic agents modulating visceral sensation. Experiment Overall Design: Three separate experiments were performed. First, 5 whole dorsal root ganglia were compared to 7 whole nodose ganglia. Second, Laser captured visceral neurons derived from 5 dorsal root ganglia and 5 nodose ganglia were compared on MG-U74Av2. Third, Laser captured visceral neurons derived from 9 dorsal root ganglia and 11 nodose ganglia were compared on Mouse430_2.

Dataset Information

Desciption of the transcriptome profiles of mouse heart innervating DRG and Nodose Ganglia-derived sensory neurons

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets