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Induced pluripotent stem cell-derived cells model brain microvascular endothelial cell glucose metabolism

ABSTRACT: To investigate the validity of using iPSC-derived brain microvascular endothelial-like cells (BMEC) as a model of human BMEC metabolism, we ran RNA-sequencing on iPSC-derived BMEC and compared gene expression of glycolytic and TCA enzymes to publically avaliable primary BMEC datasets.

ORGANISM(S): Homo sapiens

PROVIDER: GSE211648 | GEO | 2022/08/22

REPOSITORIES: GEO

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Project description:The blood-brain barrier (BBB) is lined by brain microvascular endothelial cells (BMECs) which regulate transport into and out of the brain. We used human induced pluripotent stem cell (iPSC)-derived cell types to model BBB function within 2D and 3D in vitro models. We compare gene expression between different iPSC-derived cell types, and specifically profile the response of iPSC-derived BMEC-like cells (iBMECs) to differentiation media volume, microenvironmental cues, and cytokines.

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mRNAseq of Huntington's disease and control patient iPSC-derived brain microvascular endothelial cells

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Project description:Brain microvascular endothelial cell (BMEC) injury can affect neuronal survival by modulating immune responses through the microenvironment. We used microarrays to detail the miRNAs expression in the exosomes from normal and oxygen glucose deprivation (OGD)-cultured BMECs.

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RNA sequencing of human iPSC-derived brain microvascular endothelial cells.

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Project description:Huntington's disease (HD) is associated with dysfunction of the blood-brain barrier, including brain microvasscular endothelial cells (BMECs). We report changes in gene expression of induced pluripotent stem cells (iPSCs) and iPSC-derived brain microvascular endothelial cell-like cells (iBMECs) derived from an isogenic pair of iPSCs with either 180 (HD-corrected) or 18 (HD180) CAG repeats in the HTT gene.

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