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Expression profile of TTHA1438-deficient Thermus thermophilus HB8 strain

ABSTRACT: In order to find function of TTHA1438 protein from T. thermophilus HB8, we observed the expression profile of the total mRNA of the TTHA1438-deficient strain grown in a rich medium at 70°C for 240, 360, 480, 600 and 760 min.

ORGANISM(S): Thermus thermophilus HB8

PROVIDER: GSE21198 | GEO | 2010/10/15

SECONDARY ACCESSION(S): PRJNA129459

REPOSITORIES: GEO

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Expression profile of TTHA1438-deficient Thermus thermophilus HB8 strain

Project description:In order to find function of TTHA1438 protein from T. thermophilus HB8, we observed the expression profile of the total mRNA of the TTHA1438-deficient strain grown in a rich medium at 70Â°C for 240, 360, 480, 600 and 760 min. Two TTHA1438-deficient T. thermophilus HB8 strains were each pre-cultured at 70Â°C for 16 h in 3 ml of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 ml) were inoculated into 1 liter of the same medium and then cultivated at 70Â°C. Cells were collected after 240, 360, 480, 600, 680 and 760 min, respectively, and then, the expression of each mRNA was analyzed.

2010-10-15 | E-GEOD-21198 | biostudies-arrayexpress

Time course of the mRNA expression in wild-type Thermus thermophilus HB8 strain grown on a rich medium.

Project description:We observed the expression profile of the total mRNA of wild-type Thermus thermophilus HB8 strain grown in a rich (TR) medium at 70Â°C. Keywords: time course Three wild-type T. thermophilus HB8 strains were pre-cultured at 70Â°C for 16 h in 3 ml of TR medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 ml) were inoculated into 1 liter of the same medium and then cultivated at 70Â°C. Cells were collected after 180, 240, 300, 360, 420, 480, 540, 600, 680, and 760 min, and then crude RNA was extracted. The expression of each mRNA at each time point was analyzed on a GeneChip as described under Sample Description Sheet of each sample in this website.

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Time course of the mRNA expression in wild-type Thermus thermophilus HB8 strain grown on a rich medium (labeling:ENZO)

Project description:We observed the expression profile of the total mRNA of wild-type Thermus thermophilus HB8 strain grown in a rich (TT) medium at 70oC. Keywords: time course Three wild-type T. thermophilus HB8 strains were pre-cultured at 70oC for 16 h in 3 ml of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 ml) were inoculated into 1 liter of the same medium and then cultivated at 70oC. Cells were collected after 180, 240, 300, 360, 420, 480, 540, 600, 680, and 760 min, and then crude RNA was extracted. The expression of each mRNA at each time point was analyzed on a GeneChip as described under Sample Description Sheet of each sample in this website.

2010-05-27 | E-GEOD-7165 | biostudies-arrayexpress

Time course of the mRNA expression in wild-type Thermus thermophilus HB8 strain grown in a rich medium (labeling:ENZO)

Project description:In order to observe altered expression profile of wild-type T. thermophilus HB8 strain grown in a rich (TT) medium at 70Â°C. we analyzed time-dependent mRNA expression profile of the total mRNA from the strain, which corresponds the logarithmic phase to the early stationary phase. Three wild-type T. thermophilus HB8 strains were pre-cultured at 70Â°C for 16 h in 3 ml of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 ml) were inoculated into 1 liter of the same medium and then cultivated at 70Â°C. Cells were collected after 180, 240, 300, 360, 420, 480, 540, 600, 680, and 760 min, and then crude RNA was extracted. The expression of each mRNA at each time point was analyzed on a GeneChip.

2010-10-15 | E-GEOD-21473 | biostudies-arrayexpress

Time course of the mRNA expression in wild-type Thermus thermophilus HB8 strain grown in a rich medium (labeling:Affymetrix)

2010-10-15 | E-GEOD-21290 | biostudies-arrayexpress

Expression profile of TTHB186-deficient Thermus thermophilus HB8 strain

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Expression profile of TTHA1359-deficient Thermus thermophilus HB8 strain

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Expression profile of TTHA0175-deficient Thermus thermophilus HB8 strain

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Expression profile of TTHB186-deficient Thermus thermophilus HB8 strain

Project description:In order to find function of TTHB186 protein from T. thermophilus HB8, we observed the expression profile of the total mRNA of the TTHB186-deficient strain grown in a rich medium at 70Â°C for 360, and 480 min. Three TTHB186-deficient T. thermophilus HB8 strains were each pre-cultured at 70Â°C for 16 h in 3 mL of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 mL) were inoculated into 500 mL of the same medium and then cultivated at 70Â°C. Cells were collected after 360, and 480 min, respectively, and then, the expression of each mRNA was analyzed.

2010-10-15 | E-GEOD-21457 | biostudies-arrayexpress

Expression profile of TTHA0175-deficient Thermus thermophilus HB8 strain

Project description:In order to find function of TTHA0175 protein from T. thermophilus HB8, we observed the expression profile of the total mRNA of the TTHA0175-deficient strain grown in a rich medium at 70Â°C for 300, 480, and 600 min. Two TTHA0175-deficient T. thermophilus HB8 strains were each pre-cultured at 70Â°C for 16 h in 3 ml of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 ml) were inoculated into 1 liter of the same medium and then cultivated at 70Â°C. Cells were collected after 300, 480, and 600 min, respectively, and then, the expression of each mRNA was analyzed.

2010-10-15 | E-GEOD-21471 | biostudies-arrayexpress

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