Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Growth Hormone (GH) Treatment of 3T3-F442A Adipocytes

ABSTRACT: Expression profiling of 3T3-F442A adipocytes treated with growth hormone (GH, 500 nM) or vehicle (DMEM + 1% BSA) control for 30 min., 4 hr., or 48 hr in three independent experiments. Chronic GH treatment induces metabolic changes consistent with insulin resistance in 3T3-F442A adipocytes. Keywords: time-course

ORGANISM(S): Mus musculus

PROVIDER: GSE2120 | GEO | 2005/12/20

SECONDARY ACCESSION(S): PRJNA91725

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Transcription profiling of mouse 3T3-F442A adipocytes treated with or without growth hormone (GH) to identify a mechanism for regulation of activating transcription factor 3 by GH

Project description:Expression profiling of 3T3-F442A adipocytes treated with growth hormone (GH, 500 nM) or vehicle (DMEM + 1% BSA) control for 30 min., 4 hr., or 48 hr in three independent experiments. Chronic GH treatment induces metabolic changes consistent with insulin resistance in 3T3-F442A adipocytes.

2007-09-21 | E-GEOD-2120 | biostudies-arrayexpress

Glucocorticoid target genes in 3T3-L1 adipocytes

Project description:Analysis of 3T3-L1 adipocytes treeated with dexamethasone (Dex) for 6 hours. Dex is a synthetic glucorticoid (GC) receptor agonist. Results provide insight into the effect of glucocorticoids on adipocytes. Differentiated 3T3-L1 adipocytes were cultured in DMEM supplemented with 5% stripped FBS for 24 hours and then treated with 500 nΜ Dex or an equal volume (0.05% v/v of media) of ethanol as a vehicle control for 6 hours at 37°C in DMEM with 190 5% stripped FBS. Total cellular RNA was isolated utilizing the NucleoSpin RNA II kit (Macherey-Nagel).RNA isolates were first quantified by standard spectrophotometry, and then qualitatively evaluated by capillary electrophoresis employing the Bio-Rad Experion system per the manufacturer’s instruction. The final labeled cRNA samples were hybridized overnight to Illumina mouseWG-6 BeadChip arrays, which was performed at UCSF Genomic Core. All treatments were done in triplicates and the same batch of microarrays were used for all treatments. The Illumina expression arrays were pre-processed using lumi package. The differential expression analysis was performed using the Limma package.

2010-09-15 | E-GEOD-24105 | biostudies-arrayexpress

Glucocorticoid target genes in 3T3-L1 adipocytes

2010-09-15 | GSE24105 | GEO

RNA-sequencing of 3T3-L1 preadipocytes and adipocytes

Project description:RNA-sequencing of 3T3-L1 preadipocytes and adipocytes

2022-05-12 | GSE185493 | GEO

RNAseq analysis of 3T3-L1 adipocytes cultured in high and low media volumes

Project description:3T3-L1 fibroblasts were cultured in high glucose DMEM supplemented with 10% foetal bovine serum (FBS) and 2 mM glutamax at 37°C in 10% CO2. For differentiation into adipocytes, fibroblasts were seeded into 12-well plates, and cultured in 1 mL/well of 10% FBS-supplemented DMEM containing an adipogenic cocktail (350 nM insulin, 0.5 mM 3-isobutyl-1-methylxanthine, and 250 nM dexamethasone) for 3 d, followed by 3 d in DMEM containing 10% FBS and 350 nM insulin. On day 9 post-differentiation, media volumes were either kept at 1 mL (high) or switched to 0.33 mL (low) for 16 h. Cells were then lysed in 350 µL RLT buffer for RNA extraction and sequencing.

2023-10-01 | E-MTAB-12298 | biostudies-arrayexpress

Murine 3T3-F442A Cells: Control vs. Growth hormone

Project description:Growth hormone-treated mouse 3T3-F442A adipocytes comparing control untreated F442A cells.

2016-09-13 | GSE86830 | GEO

Whole genome bisulfite sequencing of 3T3-L1 preadipocytes and adipocytes

Project description:Examination of 2 samples derived from 3T3-L1 preadipocytes and adipocytes

2022-05-12 | GSE186340 | GEO

Gene expression profile after TRPV4 knockdown in 3T3-F442A adipocytes

Project description:Comparing gene expression profile in 3T3-F442A adipocytes with shRNA against TRPV4 or GFP. TRPV4 is an ion channel expressed in adipocytes. Results provided information that how loss-of-function of TRPV4 affects gene expression in adipocytes.

2012-10-12 | GSE40280 | GEO

Gene expression signatures in IRF7 overexpressed 3T3-L1 adipocytes

Project description:To identify the genes that are regulated by IRF7, we have performed DNA microarray in 3T3-L1 adipocytes differentiated from precursor cells infected with retrovirus empty or carrying IRF7. Plasmids (Empty- and IRF7-pMSCV) were kindly provided from Dr. Eguchi at the Okayama University Graduate School of Medicine, Okayama, Japan [Cell Metab. 2008;7: 86-94.]. Infected 3T3-L1 preadipocytes were selected by puromycin treatment and differentiated into adipocytes. 7 days after the induction of adipogenesis, total RNA was isolated.

2020-04-01 | GSE147857 | GEO

The effects of adenine-midiated purinergic signaling in 3T3 fibroblasts and adipocytes

Project description:In previous study, we demonstrated that adenine could activate AMPK signaling in 3T3 fibroblast. To investigate the changes of transcriptome in differential 3T3 cells by adenine stimulation, we used microarrays to analyze undifferentited annd differentiated 3T3-L1 adipocytes in mRNA levels.

2017-02-07 | GSE94539 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data