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Expression data from an ASCL1 siRNA experiment

ABSTRACT: To elucidate whether or not a subtype of adenocarcinoma with neuroendocrine nature has poor prognosis, we performed gene expression profiling of an achaete-scute complex homolog 1 (ASCL1) siRNA experiment.

ORGANISM(S): Homo sapiens

PROVIDER: GSE21216 | GEO | 2011/07/19

SECONDARY ACCESSION(S): PRJNA126313

REPOSITORIES: GEO

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Expression data from an ASCL1 siRNA experiment

Project description:To elucidate whether or not a subtype of adenocarcinoma with neuroendocrine nature has poor prognosis, we performed gene expression profiling of an achaete-scute complex homolog 1 (ASCL1) siRNA experiment. siRNA againt ASCL1 was transfected into DMS79 cells.

2011-07-18 | E-GEOD-21216 | biostudies-arrayexpress

Expression profiling of Ascl1-reprogrammed P12 Müller glia compared with freshly dissociated progenitors and Müller glia

Project description:Non-mammalian vertebrates have a robust ability to regenerate injured retinal neurons from Müller glia cells (MG) that activate the proneural factor Achaete-scute homolog 1 (Ascl1/Mash1) and de-differentiate into progenitors cells. In contrast, mammalian MG have a limited regenerative response and fail to upregulate Ascl1 after injury. To test whether Ascl1 could restore a neurogenic potential to mammalian MG, we over-expressed Ascl1 in dissociated mouse MG cultures and intact retinal explants. Ascl1-infected MG upregulate retinal progenitor-specific genes, while downregulating glial genes. Furthermore, Ascl1 remodeled the chromatin at its targets from a repressive to active configuration. MG-derived progenitors differentiated into cells that exhibited neuronal morphologies, expressed retinal subtype-specific neuronal markers, and displayed neuron-like physiological responses. These results indicate that a single transcription factor, Ascl1, can produce a neurogenic state in mature Muller glia. Expresssion profiling was used to determine the genes that were changed after Ascl1 infection of P12 cultured Müller glia compared with those present in P0 progenitors and P7-P21 Müller glia Retinas were dissociated and FAC-sorted from Hes5-GFP mice at P0, P7, P10, P14 or P21 and submitted for profiling. WT Retinas were dissociated at P12, grown for 1 week in culture, and infected with lentiviruses expressing Ascl1 or GFP for four days. Total RNA was extracted and submitted for profiling.

2013-04-06 | E-GEOD-45835 | biostudies-arrayexpress

Expression profiling of Ascl1-reprogrammed P12 Müller glia compared with freshly dissociated progenitors and Müller glia

2013-04-06 | GSE45835 | GEO

Ascl1

Project description:Rattus norvegicus Ascl1, achaete-scute family bHLH transcription factor 1 [Source:RGD Symbol;Acc:71010], is differentially expressed in 6 experiment(s);

| ENSRNOG00000004294 | ExpressionAtlas

Ascl1

Project description:Rattus norvegicus Ascl1, achaete-scute family bHLH transcription factor 1 [Source:RGD Symbol;Acc:71010], is expressed in 3 baseline experiment(s);

| ENSRNOG00000004294 | ExpressionAtlas

ASCL1

Project description:Xenopus tropicalis ASCL1, achaete-scute family bHLH transcription factor 1 [Source:HGNC Symbol;Acc:HGNC:738], is differentially expressed in 1 experiment(s);

| ENSXETG00000041303 | ExpressionAtlas

ASCL1

Project description:Monodelphis domestica ASCL1, achaete-scute family bHLH transcription factor 1 [Source:HGNC Symbol;Acc:HGNC:738], is expressed in 1 baseline experiment(s);

| ENSMODG00000041211 | ExpressionAtlas

ASCL1

Project description:Equus caballus ASCL1, achaete-scute family bHLH transcription factor 1 [Source:HGNC Symbol;Acc:HGNC:738], is expressed in 1 baseline experiment(s);

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ASCL1

Project description:Homo sapiens ASCL1, achaete-scute family bHLH transcription factor 1 [Source:HGNC Symbol;Acc:HGNC:738], is expressed in 31 baseline experiment(s);

| ENSG00000139352 | ExpressionAtlas

ASCL1

Project description:Macaca mulatta ASCL1, achaete-scute family bHLH transcription factor 1 [Source:VGNC Symbol;Acc:VGNC:70029], is expressed in 2 baseline experiment(s);

| ENSMMUG00000005747 | ExpressionAtlas

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