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High resolution definition of the Staphylococcus aureus WalKR regulon

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Staphylococcus aureus

PROVIDER: GSE212321 | GEO | 2022/09/01

REPOSITORIES: GEO

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High resolution definition of the Staphylococcus aureus WalKR regulon [RNA-seq]

Project description:Here, we use a panel of defined walKR mutants and functional genomics including ChIP-seq to confirm a high-resolution consensus WalR DNA-binding motif and characterise a 61-gene direct regulon

2022-09-01 | GSE212319 | GEO

High resolution definition of the Staphylococcus aureus WalKR regulon [ChIP-seq]

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Transcriptome analysis of the WalKR regulon in Staphylococcus aureus

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Staphylococcus aureus HG001

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2016-03-08 | E-GEOD-70043 | biostudies-arrayexpress

Transcriptome analysis of the WalKR regulon in Staphylococcus aureus

Project description:The WalKR regulon was studied by comparing genes expression in a strain producing a constitutively active WalR regulator (WalRc) versus the wild type strain carrying the empty expression vector. Hybridizations were performed with three independent biological replicates. Our data allowed to identify 165 genes differentially expressed in the walRc expressing strain with a P-value M-bM-^IM-$ 0.05 using Z-test and a threshold value of two-fold change in transcriptional levels. Among them, 108 genes are activated, and 57 genes are repressed. About 10% of these results were confirmed by quantitative real time PCR, revealing the high reliability of this overall study. Beyond the major effect of the WalKR system on genes expressing cell wall hydrolases, we have shown that it activates a large number of genes involved in virulence, and probably through its positive impact on the SaeRS two-component system, is a major activator of S. aureus virulence. Cells were grown in TSB rich medium added with 0.25 M-BM-5M CdCl2 to allow walRc expression until OD600nm of 1. Gene expression was compared between the HG001 wild type reference strain carrying the pCN51 empty vector (HG001/pCN51) and the HG001 strain carrying a pCN51 derivative with the walRc allele under the control of the Pcad inducible promoter (HG001/pSD3-12). Each condition was done in triplicate and the data were analyzed using the Arraystat software. Genes were considered as differentially expressed if their transcriptional level differed by at least two-fold with a Z-test P-value M-bM-^IM-$ 0.05 in at least two of the replicates.

2012-09-13 | E-GEOD-29337 | biostudies-arrayexpress

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SarA regulon in S. aureus

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Genome-wide Definition of the SigF Regulon in Mycobacterium tuberculosis

Project description:This SuperSeries is composed of the SubSeries listed below.

2012-01-20 | GSE35080 | GEO