Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effect of aging and dFOXO knockout on small RNAs

ABSTRACT: To examine the effect of loss of dFOXO on the small RNA landscape of aging animals, we sequenced total small RNA collected from whole wildtype (wDah) and dFOXO-null (dfoxoΔ94) flies at young (5-6 days) and old (31 days) age.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE212493 | GEO | 2022/09/04

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Effect of aging and dFOXO knockout on small RNA in Ago1 and Ago2 RNA-induced silencing complexes

Project description:To examine the effect of loss of dFOXO on Ago1 and Ago2 RISC loading in aging animals, we sequenced RNA immunoprecipitated from Ago1 and Ago2 RISC collected from whole wildtype (wDah) and dFOXO-null (dfoxoΔ94) flies at young (5 days) and old (35 days) age. dFOXO is a transcription factor that regulates Ago1 and Ago2 as well as lifespan.

2022-09-04 | GSE212492 | GEO

Feedback from dFOXO in the gut and fat body

Project description:Whole fly transcript profiles of changes upon induction of dFOXO in the gut and fat body using S1106>dFOXO flies and how this response changes when the feedback to foxo is removed in the foxo null background

2014-02-05 | E-MTAB-1232 | biostudies-arrayexpress

dFOXO targets in Drosophila melanogaster

Project description:dFOXO targets in adult Drosophila melanogaster females, and the effect of insulin signalling and stress on binding. The experimets determined the binding locations of dFOXO in the whole adult female fly using ChIP-chip. The protocol was validated using mock conditions: pre-immune serum or IP on chromatin from foxo null flies. The response of this binding to stress induced by treatment of flies with paraquat or by their exposure to starvation, as well as the response to an insulin-signalling-reducing genetic manipulation (over-expression of dominant negative form of the insulin receptor), was determined.

2011-06-18 | E-TABM-751 | biostudies-arrayexpress

Genome-wide analysis of dFOXO binding sites in Drosophila

Project description:To understand how reduced insulin/IGF-1 signaling extends Drosophila lifespan through its downstream transcription factor dFOXO. We conducted ChIP analysis with a dFOXO antibody followed by Illumina high-throughput sequencing from chico heterozygous mutants, which are long-lived and normal sized, and from adult flies with ablated insulin producing cells (IPCs), which are also long-lived. dFOXO bound at promoters of 273 genes common among these genotypes, thus potentially enriching for shared factors in control of aging. Two replicates were sequenced from chico heterozygous mutants and IPC ablated flies.

2013-09-20 | E-GEOD-44686 | biostudies-arrayexpress

Genome-wide analysis of dFOXO binding sites in Drosophila

2013-09-20 | GSE44686 | GEO

Transcriptional analysis of dInR and dfoxo epistasis in Drosophila melanogaster

Project description:Transcriptional analysis of dInR and dfoxo epistasis in Drosophila melanogaster. The experiment was performed to examine which parts of the transcriptional response to a reduction in insulin signalling in an adult female fly depend on the presence of the dfoxo transcription factor. Whole fly transcriptome was determined with flies over-expressing a dominant negative form of the insulin receptor, or the wild-type fly, in presence or absence of dfoxo.

2011-06-18 | E-TABM-757 | biostudies-arrayexpress

Transcriptional memory of dFOXO activity in early adulthood delays late life mortality [RNA-seq]

Project description:The study examined transcriptional memory after dFOXO induction in fat body and gut of young adult female fruit flies.

2022-10-06 | GSE183541 | GEO

Transcriptional memory of dFOXO activity in early adulthood delays late life mortality [ATAC-seq]

Project description:The study examined transcriptional memory after dFOXO induction in fat body and gut of young adult female fruit flies.

2022-10-06 | GSE183540 | GEO

Transcription profiling by array of adult Drosophila melanogaster gut and fat body in S1106>dfoxo females after 5 days of induction of transgene expression by RU486 feeding.

Project description:Transcription profiling by array of adult abdominal/thorasic fat body or midgut in S1106>dfoxo females after 5 days of induction of transgene expression by RU486 feeding.

2014-08-18 | E-MTAB-1020 | biostudies-arrayexpress

Drosophila brain gene expression between wildtype and miR-34 null flies

Project description:gene expression profiles in fly brains between wildtype and miR-34 null flies gene expression profiles in fly brains, wild type (3d, 20d) and miR-34 null flies (3d, 20d)

2011-12-23 | E-GEOD-25008 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data