Dataset Information

Genome-wide analysis of histone trimethylation in INMA cohort exposed to BPA revealed a global impact on telomeric binding proteins and histone acetyltransferase factors

ABSTRACT: This study was developed under the framework of the Human Biomonitoring for Europe Initiative (HBM4EU). We evaluated the possible impacts of BPA exposure in adolescent males from the INMA-Granada cohort (Spain) with the goal of identifying new biomarkers that could be useful in biomonitoring and epidemiological studies. Our hypothesis is that alterations in epigenetic regulation could lead to genetic instability in human subjects exposed to environmentally relevant doses of BPA.

ORGANISM(S): Homo sapiens

PROVIDER: GSE212713 | GEO | 2022/11/16

REPOSITORIES: GEO

ACCESS DATA

Similar Datasets

Project description:We used the rhesus monkey (Macaca mulatta) as our animal model for the current study with two goals: to characterize the changes in histology and gene expression from early to late gestation (prenatal uterine organogenesis) and to determine if there are effects of prenatal exposure to bisphenol A (BPA) on the developing female uterus. Pregnant rhesus monkeys carrying a female fetus (N=22) were divided into two experimental groups, based on gestational timing: 'early' (N=10) and 'late' (N=12). These groups were then equally sub-divided into control (unexposed) and BPA (exposed) groups (5 Early Control, 5 Early BPA-exposed, 6 Late Control and 6 Late BPA-exposed.) The BPA-exposed monkeys received a deuterated BPA (dBPA, CDN Isotopes, Quebec, Canada) fruit treat on a daily basis, at a dose of 400ug/kg/day). The dosing was aimed at achieving serum levels of BPA detected in adult human biomonitoring studies. The control animals received a vehicle control on a daily basis. The 'early' time period (mid-gestation) referred to gestational days 50-100, approximating the second trimester of human gestation. The fetal monkeys in the 'early' group were delivered via cesarean section on gestation day 100 and euthanized. Samples of maternal and fetal blood and amniotic fluid were obtained. Maternal and fetal weights were also recorded. The 'late' time period referred to gestational days 100-165, approximating the third trimester of human gestation. The fetal monkeys in the 'late' group were delivered vaginally and euthanized. There were five idiopathic stillbirths (2 Control, 3 BPA-exposed) in the late group. Samples of maternal and fetal blood and amniotic fluid were obtained. Maternal and fetal weights were also recorded. After delivery, the fetal uterus was excised and cut sagitally from fundus to cervix; one side was fixed for histological evaluation and the other half was frozen for analysis of gene expression by microarray. The stillbirths were excluded from the microarray.

Dataset Information

Genome-wide analysis of histone trimethylation in INMA cohort exposed to BPA revealed a global impact on telomeric binding proteins and histone acetyltransferase factors

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure