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Expression analysis of Thermus thermophilus HB8 wild-type strain in response to high growth temperature

ABSTRACT: In order to observe the heat-shock response of extremely thermohilic bacterium T. thermophilus HB8 strain, we analyzed the altered expression profile of the total mRNA in T. thermophilus HB8 wild-type strain after the growth temperature was shifted from 70°C to 80°C for 30 min.

ORGANISM(S): Thermus thermophilus HB8

PROVIDER: GSE21288 | GEO | 2010/10/15

SECONDARY ACCESSION(S): PRJNA129441

REPOSITORIES: GEO

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Expression analysis of Thermus thermophilus HB8 wild-type strain in response to high growth temperature

Project description:In order to observe the heat-shock response of extremely thermohilic bacterium T. thermophilus HB8 strain, we analyzed the altered expression profile of the total mRNA in T. thermophilus HB8 wild-type strain after the growth temperature was shifted from 70Â°C to 80Â°C for 30 min. Three wild-type strain of T. thermophilus HB8 were pre-cultured at 70Â°C for 16 hours in 3 mL of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (0.5 mL) were inoculated into 250 mL of TT medium and then cultivated at 70Â°C for 6 hours. After the cells were collected from 50 ml culture medium, the remaining medium was cultured at 80Â°C for 30 min. The cells were collected, and then crude RNA was extracted from each lot of cells. The expression of each mRNA at each time point was analyzed on a GeneChip.

2010-10-15 | E-GEOD-21288 | biostudies-arrayexpress

mRNA expression in wild type and TTHA0175-deficient Thermus thermophilus HB8 strains; rich medium with growth temperature shift from 70C to 45C

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Expression profile of crp-deficient Thermus thermophilus HB8 strain

Project description:We observed the expression profile of the total mRNA of crp (TTHA1437) deletion mutant of Thermus thermophilus HB8 strain grown in a rich (TT) medium at 70 degC

2010-10-15 | GSE21459 | GEO

Expression data from Thermus thermophilus HB8 wild type and TTHA1559 disrupted

Project description:We observed the expression profile of the total mRNA of wild-type and TTHA1559 disrupted Thermus thermophilus HB8 strain at 70°C.

2014-08-03 | GSE33985 | GEO

TTHB212 (anti-sigmaE)-deficient Thermus thermophilus HB8 strain: time course

Project description:We observed the expression profile of the total mRNA of TTHB212-deficient of Thermus thermophilus HB8 strain grown in a rich medium at 70°C for 7 and 8 hours

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Expression profile of TTHA0101-deficient Thermus thermophilus HB8 strain

Project description:In order to find function of TTHA0101 protein from T. thermophilus HB8, we observed the expression profile of the total mRNA of the TTHA0101-deficient strain grown in a rich medium at 70°C for 680 min.

2010-10-15 | GSE21453 | GEO

Expression profile of TTHA0167-deficient Thermus thermophilus HB8 strain

Project description:In order to find function of TTHA0167 protein from T. thermophilus HB8, we observed the expression profile of the total mRNA of the TTHA0167-deficient strain grown in a rich medium at 70°C for 480 min.

2010-10-15 | GSE21617 | GEO

Expression profile of TTHA1431-deficient Thermus thermophilus HB8 strain

Project description:In order to find function of TTHA1431 protein from T. thermophilus HB8, we observed the expression profile of the total mRNA of the TTHA1431-deficient strain grown in a rich medium at 70°C for 360 min.

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Expression profile of TTHA0845-deficient Thermus thermophilus HB8 strain

Project description:In order to find function of TTHA0845 protein from T. thermophilus HB8, we observed the expression profile of the total mRNA of the TTHA0845-deficient strain grown in a rich medium at 70°C for 420 min.

2010-10-15 | GSE21461 | GEO

Expression profile of TTHA0995-deficient Thermus thermophilus HB8 strain

Project description:In order to find function of TTHA0995 protein from T. thermophilus HB8, we observed the expression profile of the total mRNA of the TTHA0995-deficient strain grown in a rich medium at 70°C for 680 min.

2010-10-15 | GSE21197 | GEO

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