Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Inter-organellar communication by a mitochondrial tRNA regulates nuclear metabolic transcription (RNA-Seq)

ABSTRACT: To assess transcriptomic changes that occur upon mito-tRNA Asparagine inhibition, MDA-MB-231 cells were transfected with either a non-targeting control locked nucleic acid (LNA), two independent LNAs targeting mito-tRNA Asparagine, or an LNA against mito-tRNA Lysine.

ORGANISM(S): Homo sapiens

PROVIDER: GSE213171 | GEO | 2023/07/01

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Inter-organellar communication by a mitochondrial tRNA regulates nuclear metabolic transcription (GRO-Seq and CUT&RUN)

Project description:To assess changes in HDAC2 genomic occupancy an transcription rate that occur upon mito-tRNA Asparagine inhibition, MDA-MB-231 cells were transfected with either a non-targeting control locked nucleic acid (LNA), or an LNA targeting mito-tRNA Asparagine. HDAC2-bound loci were assessed using CUT&RUN, while transcriptional activity was assessed using GRO-Seq

2023-07-01 | GSE213170 | GEO

YBX1-dependent LNA-tRF-mediated modulation of gene expression

2015-05-08 | E-GEOD-63564 | biostudies-arrayexpress

YBX1-dependent LNA-tRF-mediated modulation of gene expression under hypoxia

Project description:In order to test whether inhibition of tRFs induced under hypoxia would counteract the YBX1-dependent reduction of target transcripts, we used transient transfection of antisense locked-nucleic acids (pooled LNAs) against tRFAsp, tRFGly, tRFGlu, and tRFTyr under hypoxia followed by microarray profiling. Synthetic antisense locked-nucleic acids (LNAs) targeting the YBX1 binding site on tRFAsp, tRFGly, tRFGlu, and tRFTyr were transfected (as a pool) in control and YBX1-knockdown cells under hypoxia to assess the consequences of inhibiting tRFs induced in hypoxic MDA-parental cells.

2015-05-08 | E-GEOD-63563 | biostudies-arrayexpress

YBX1-dependent LNA-tRF-mediated modulation of transcript stability

Project description:In order to identify YBX1-dependent targets that are modulated upon changing the levels of endogenous tRFs, we used transient transfection of antisense locked-nucleic acids (LNAs) against tRFAsp, tRFGly, tRFGlu, and tRFTyr followed by alpha-amanitine treatment, RNA extraction at time points 0 and 8hr post-treatment, and transcriptomic profiling. Synthetic antisense locked-nucleic acids (LNAs) targeting the YBX1 binding site on tRFAsp, tRFGly, tRFGlu, and tRFTyr were transfected into control and YBX1-knockdown cells to identify YBX1-dependent targets whose stabilities are modulated due to tRF loss-of-function. We used alpha-amanitine mediated inhibition of RNA-polymerase to measure transcript stability across the entire transcriptome.

2015-05-08 | E-GEOD-63565 | biostudies-arrayexpress

Study of the role of miR-205 in prostate epithelial cells

Project description:Gene expression has been assessed on RWPE-1 cells, derived from normal prostate, upon silencing of miR-205 by an antisense locked nucleic acid. RWPE-1 cells were transfected in triplicate either with 100nM of an antisense locked nucleic acid targeting miR-205 (LNA-205) or a scrambled oligomer (LNA-Scr). Gene expression was assessed 72h after transfection.

2012-06-11 | E-GEOD-29782 | biostudies-arrayexpress

Inter-organellar communication by a mitochondrial tRNA regulates nuclear metabolic transcription

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-07-01 | GSE213173 | GEO

GEP data from BCWM.1 cells treated with LNA antimiR-155 or scramble control

Project description:microRNA-155 acts as an oncogenic miRNA in B-cell lymphoproliferative disorders including Waldenstrom Macroglobulinemia (WM) and Chronic Lymphocytic Leukemia (CLL). we used an 8-mer LNA (locked nucleic acid) phosphorothioate oligonucleotide targeting the seed region of miR-155 to effectively antagonize in vitro tumor growth in WM. We performed gene expression profiling (GEP) analysis on BCWM.1 cells after treatment with LNA antimiR-155 or scramble control for 48 hours.

2012-07-09 | E-GEOD-39189 | biostudies-arrayexpress

YBX1-dependent LNA-tRF-mediated modulation of gene expression

2015-05-08 | GSE63564 | GEO

GEP data from BCWM.1 cells treated with LNA antimiR-155 or scramble control

2012-07-10 | GSE39189 | GEO

YBX1-dependent LNA-tRF-mediated modulation of gene expression under hypoxia

2015-05-08 | GSE63563 | GEO