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Lactoferrin attenuated deoxynivalenol induced malfunction in mouse testis

ABSTRACT: DON impaired the male spermatogenesis and induced oxidative stress, testicular apoptosis and disruption of blood-testis barrier (BTB). The milk protein lactoferrin (LF) is an iron-binding glycogenprotein with multifunctions including anti-inflammation and antioxidant. The current study was aimed to investigate the beneficial effects of LF on spermatogenesis and integray of BTB of mice treated with diet DON.

ORGANISM(S): Mus musculus

PROVIDER: GSE213641 | GEO | 2023/01/06

REPOSITORIES: GEO

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Transcriptome analysis reveals the mechanism of bovine lactoferrin against lipopolysaccharide-induced acute lung injury

Project description:Lactoferrin (LF) is an iron-binding multifunctional cationic glycoprotein secreted by exocrine glands and neutrophils in mammals. In this study, we created a mouse model of lipopolysaccharide (LPS)-induced acute lung injury and explored the anti-inflammatory effect and mechanism of bovine lactoferrin (bLF) in acute lung injury using the RNA sequencing (RNA-seq) technology and transcriptome analysis.

2023-12-19 | GSE247266 | GEO

Transcriptomics analysis of amphotericin B and lactoferrin drug synergy in Cryptococcus neoformans H99

Project description:Invasive fungal infections (IFIs) are difficult to treat. Few effective antifungal drugs are available and many have problems with toxicity, efficacy and drug-resistance. To overcome these challenges, existing therapies may be enhanced using more than one agent acting in synergy. Previously, we have found amphotericin B (AMB) and the iron chelator, lactoferrin (LF), were synergistic against Cryptococcus neoformans and Saccharomyces cerevisiae. This study investigates the mechanism of AMB+LF synergy using RNA-seq in Cryptococcus neoformans H99.

2019-09-08 | GSE130375 | GEO

Global transcriptome analysis of paracancerous lung tissues from the B16 metastasis mice model under the influence of lactoferrin knockout

Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are using transcriptome profiling (RNA-seq) to evaluate the effects of lactoferrin deficiency on the global transcriptome of the lung microenvironment of the B16 metastasis mice model. Methods: 2×10^5 B16-F10 cells were injected into WT and lactoferrin kncoutout (Lf-/-) mice (each group has 10 eight-weeks-old male mice) through tail vein. At 3 weeks post injection,the paracancerous lung tissue were isolated (not including any metastatic colonies). RNAs were extracted by Trizol and sequenced by Solexa high-throughput sequencing service (Oebiotech, Shanghai, China). Data were extracted and normalized according to the manufacturer’s standard protocol.Each group has two mice paracancerous lung tissues be tested. Results: There are significant gene expression profile changes between WT and Lf-/- tumor-bearing mice. Conclusions: Our study describes the global transciptome changes of paracancerous lung tissues from the B16 metastasis mice model under the influence of lactoferrin kncokout.

2019-12-31 | GSE120179 | GEO

Expression data from postnatal piglet hippocampus, investigating how lactoferrin benefit early neurodevelopment and cognition

Project description:Lactoferrin supplementation in pig milk replacer could promote cognitive functions in postnatal piglet behaviour experiment. There are 3 groups of 3-day-old postnatal piglets, feeding basal level, medium dose(0.6g/L) and high dose(1.2g/L) lactoferrin. After 35days feeding, medium dose group showed better performance in behaviour experiment. We used microarrays to detail the global programme of gene expression underlying the nervous system development and memory formation.

2017-02-28 | GSE66317 | GEO

Functional microarray analysis of neonatal monocyte-derived macrophages differentiated in the presence or absence of human lactoferrin

Project description:Monocytes were isolated from umbilical cord blood of term born infants and differentiated with M-CSF in the presence or absence of human lactoferrin for 7 days. Afterwards cells were stimulated with LPS or left untreated, RNA isolated using a RNeasy Plus Micro Kit and samples submitted to microarray-analysis. The experiments were performed to investigate the functional impact of human lactoferrin, a transferrin glycoprotein highly prevalent in human breast-milk, on neonatal macrophages. The experimental model serves as a proxy for the proteins' immunoregulatory function within the developing neonatal gut.

2023-08-08 | E-MTAB-13175 | biostudies-arrayexpress

Gene expression profiling in HEK293 cells overexpressed lactoferrin gene that has the signal sequence (sLF) or not (ΔLF)

Project description:Human lactoferrin (LF) is a multifunctional protein involved in immunomodulation, cell growth, and differentiation. In addition to the secreted form (sLF), an alternatively spliced form (ΔLF) that lacks the signal sequence and downregulated in cancer was found. This study was carried out to identify and compare signaling networks provoked by the two LF isoforms. To do this, the two forms were overexpressed in HEK293 cells using the flp-in/tet-on system and genome-wide expression analysis of 18,367 genes was conducted.

2011-09-07 | GSE31878 | GEO

Gene expression profiling in HEK293 cells overexpressed lactoferrin gene that has the signal sequence (sLF) or not (ΔLF)

2011-09-06 | E-GEOD-31878 | biostudies-arrayexpress

Site-specific glycosylation of donkey milk lactoferrin investigated by High Resolution Mass Spectrometry

Project description:A comprehensive glycosylation profile of donkey lactoferrin, isolated by ion exchange chromatography from an individual milk sample, was obtained by means of chymotryptic digestion, TiO2 and HILIC enrichment, reversed-phase high performance liquid chromatography, electrospray mass spectrometry, and high collision dissociation fragmentation. The results obtained allowed the identification of 26 different glycan structures, including high mannose, complex and hybrid N-glycans, linked to the protein backbone via an amide bond to asparagine residues located at the positions 137, 281 and 476. Altogether, the N-glycan structures determined revealed that in donkey milk lactoferrin most of the N-glycans identified are neutral complex/hybrid. Actually, 10 neutral non-fucosylated complex/hybrid N-glycans and 4 neutral fucosylated complex/hybrid N-glycans were found. In addition, 2 high mannose N-glycans, 4 sialylated fucosylated complex/hybrid N-glycans and 6 sialylated non-fucosylatedN-glycans, one of which containing N-glycolylneuramin acid (Neu5Gc), were found. A comparison of the glycosylation profile of donkey milk lactoferrin with respect to that of human, bovine and goat milk lactoferrin is reported.

2016-08-25 | PXD004289 | Pride

Effect of lactoferrin treatment on iAEC2

Project description:In an attempt to understand the mode of antiviral action of lactoferrin on SARS-CoV-2 infection, we focused on the effect of lactoferrin on uninfected cells. We decided to use a physiologically relevant cell model of iPSC derived- alveolar epithelial cells (the iAEC2). We specifically focused on antiviral pathway triggered by lactoferrin on treated cells that could explain a cell-mediated effect of lactoferrin on viral replication

2021-07-15 | GSE171390 | GEO

Metabolomic profiling reveals the effects of early-life lactoferrin intervention on protein synthesis, energy production and antioxidative capacity in the liver of suckling piglets

Project description:<p>The objective of this study was to reveal the effects of early life lactoferrin intervention on the liver metabolism in the suckling piglets. Sixty newborn piglets with an average initial body weight (BW) of 1.51 ± 0.05 kg were assigned to the control (CON) group and the lactoferrin (LF) group. Piglets in the LF group were orally administered LF solution (0.5 g/kg BW per day) from 1 to 7 days of age, and the piglets in the CON group were orally administrated with the same dose of physiological saline. Plasma, jejunum and liver samples were collected on day 8 and 21. The LF piglets had a decreased level of plasma urea nitrogen on day 8 and an increased level of plasma albumin on day 21. Pathway analysis of metabolomic profiles showed that LF treatment affected amino acid metabolism in the liver of piglets. Additionally, LF treatment upregulated the gene expression of proteolytic enzyme and amino acid transporters (APA, aminopeptidases A; APN, aminopeptidases N; EAAC 1, excitatory amino acid carrier 1; Pept 1, peptide transporter 1) in the jejunum, and enhanced phosphorylation levels of mTOR and p70S6K in the liver. Moreover, LF treatment upregulated the expression of β oxidation related gene (CPT 1, carnitine palmitoyl transferase-1) and affected tricarboxylic acid (TCA) cycle in the liver on day 21. Furthermore, LF piglets had a lower level of MDA and a higher level of GSH and GSH-px in the liver mitochondria. Overall, the early life LF intervention affected protein synthesis, energy production and antioxidative capacity in the liver of neonatal piglets.</p>

2022-03-01 | MTBLS2279 | MetaboLights

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