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Affymetrix SNP array data for induced pluripotent stem cell line

ABSTRACT: Human induced pluripotent stem cells (iPSCs) provide a virtually inexhaustible source of starting material for cell therapies, such as next generation mesenchymal stem/stromal cells (i.e., iPSC-MSCs), offering an increased clinical efficacy in a variety of diseases. In particular, iPSC-derived products are relevant for treating pathologies in pediatric patients, due to the limited amount of biological material that is easily accessible in infants, toddlers, or young children. Despite iPSCs can be generated from many tissue sources, a non-invasive sampling approach would foster and broaden the interest in pediatric research. Here, we report the generation of two iPSC lines using a small amount of urine from pediatric patients with brain tumors (BT-iPSC). Urinary epithelial cells were isolated and reprogrammed using non-integrative Sendai virus vectors encoding the Yamanaka factors KLF4, OCT3/4, SOX2 and CMYC. After reprogramming, BT-iPSC lines were subject to quality assessment and compared to iPSCs obtained from urine samples of non-tumor pediatric patients (nonT-iPSC). We demonstrated that a small volume of urine is a non-invasive and reliable source of somatic cells to generate iPSCs from pediatric patients. In addition, we showed that BT-iPSCs are comparable to nonT-iPSCs and efficiently differentiate into iPSC-MSCs. This is an attractive approach for producing patient-specific cell products, even from those with brain tumors.

ORGANISM(S): Homo sapiens

PROVIDER: GSE213813 | GEO | 2023/02/15

REPOSITORIES: GEO

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