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Sensitive detection of multi-cancer and prediction of patient survival by methylomes of plasma cell-free DNA

ABSTRACT: Aberrant DNA methylation occurs early in tumorigenesis, and DNA methylomes of cancer cells and plasma cell free DNA (cfDNA) have been used for tumor detection and classification. Here we report a highly sensitive optimized method of methylated DNA immunoprecipitation sequencing (MeDIP-Seq) for the detection of DNA methylation of genomic DNA and cfDNA (cfMeDIP-Seq). The optimized MeDIP-Seq can generate high quality MeDIP-Seq datasets using less than 100 ng genomic DNA and 5-20 ng single-stranded cfDNA, which can generally be purified from 200-300 ul of plasma. Using the improved cfMeDIP-Seq procedures, we analyzed cfDNA methylomes of 87 samples for subjects with liver cancer, brain cancer and healthy individuals.

ORGANISM(S): Homo sapiens

PROVIDER: GSE213890 | GEO | 2025/09/01

REPOSITORIES: GEO

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