Spatial transcriptome analysis of mouse placenta in different developmental time points

ABSTRACT: Placenta is the vital reproductive organ of mouse, which is required for sustainable growth, protection, and nourishment of the fetus during gestation. It performs many crucial functions which include removal of waste products from the fetus, facilitation of the oxygen supply and nutrients to the fetus etc. Advances in the next generation sequencing technology, especially single cell transcriptional profiling, have provided the opportunity to explore the cell heterogeneity in mouse placenta. However spatial organization of these cell types and their functional mechanism, genes co-expression and regulations remain elusive yet. Visium Spatial 10x is a modern technology to study gene expression, where whole transcriptome analysis has been performed while taking account of their morphological context. This helps to gain a complete view of disease complexity, discover new biomarkers, identify novel cell types and developmental states. Along with it is used to identify spatiotemporal gene expression patterns and create map of the spatial organization of cell atlas. Placental tissue comprised of different cell types and are well organized display signature of developmental stages, along with their gene's expression. In this regard, exploring the new cell types and tracking their developmental trajectory is important to get insight into their role in spatial organizational context. Cellular functions and extensive interactions among cell types are unexplored taking consideration of spatial context in mouse placenta. It is required to explore the cell types, cell-to-cell communication which is essential for deciphering the regulation of placental development and functions. In this study, we have applied spatial transcriptomes for two developmental stages of mouse placenta E10.5 and E12.5 to explore the cell heterogeneity of the developing placenta. This work shows their significance by providing insight of tissue level identification cell types of clusters, identification of cell types of markers to get the significantly expressed genes in particular clusters. The arrangement of different cell types on tissue images are more informative to study the arrangement and cell differentiation in developmental stages. In conclusion, this study provides new insight into the development and functions of placenta.

ORGANISM(S): Mus musculus

PROVIDER: GSE214004 | GEO | 2025/09/23

REPOSITORIES: GEO