Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

EC_tagging pulse-chase to measure mRNA decay in Drosophila larval neuroblasts and neurons

ABSTRACT: Nascent RNA was tagged with EU, via EC feeding, in targeted cells (neuroblasts or neurons) then purified after the initial pulse labeling or after a chase using media containing excess unmodified uridine

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE214062 | GEO | 2022/09/26

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other
	GSE214062_insc_PC_processed.txt.gz	Txt
	GSE214062_nsyb_PC_processed.txt.gz	Txt

Items per page:

1 - 3 of 3

Similar Datasets

EC_tagging pulse-chase to measure mRNA decay in Drosophila larval neuroblasts and neurons

Project description:EC_tagging pulse-chase to measure mRNA decay in Drosophila larval neuroblasts and neurons

| PRJNA883697 | ENA

Thiouracil Pulse-chase, Tachyzoites and Bradyzoites mRNA stability data

Project description:Pulse chase measurements using thiouracil (DTU) labeling via UPRT and chasing with uracil Data from tachyzoites is labeled "DTU Pulse Chase". Two independent pulse chase experiments were performed in tachyzoites, pulse chase 1 and 2. Duplicate arrays at each timepoint were performed for pulse chase 2 (2 a and b). Data from bradyzoites are labeled "DTU Bradyzoite Pulse Chase". Two independent pulse chase experiments were performed in bradyzoites and a single set of arrays were performed for each experiment. Just one chase timepoint was used in the bradyzoite experiments, the 2 hour chase. An RNA stablity experiment design type examines stability and/or decay of RNA transcripts. Keywords: RNA_stability_design

2005-07-16 | GSE2947 | GEO

Thiouracil Pulse-chase, Tachyzoites and Bradyzoites mRNA stability data

2005-07-16 | E-GEOD-2947 | biostudies-arrayexpress

Transcription profiling of Toxoplasma gondii thiouracil pulse-chase, tachyzoites and bradyzoites mRNA stability data

2005-07-07 | E-SMDB-2949 | biostudies-arrayexpress

In vivo pulse-chase in C. elegans reveals intestinal histone turnover changes upon starvation

Project description:The ability to study protein dynamics and function in the authentic context of a multicellular organism is paramount to better understand biological phenomena in animal health and disease. Pulse-chase of self-labeling tag fusion proteins provide the opportunity to label proteins of interest and track those proteins over time. There are currently several challenges associated with performing in vivo protein pulse-chase in animals, such as cost, reproducibility, and accurate detection methods. The C. elegans model organism has attributes that alleviate many of these challenges. This work tests the feasibility of applying the Halo modified enzyme (HaloTag) for in vivo protein pulse-chase in C. elegans. HaloTag intestinal histone reporters were created in the worm and used to demonstrate that reporter protein could be efficiently pulse-labeled by soaking animals in ligand. Labeled protein stability could be monitored over time by fluorescent confocal microscopy. Further investigation revealed reporter protein stability was dependent on the animal’s nutritional state. ChIP-seq of the reporters showed incorporation in chromatin with little change hours into starvation, implying a lack of chromatin regulation at the time point tested. Collectively, this work presents a straightforward method to label and track proteins of interest in C. elegans that can address a multitude of biological questions surrounding protein stability and dynamics in this animal model.

2025-02-19 | GSE289739 | GEO

Pulse-chase SILAC analysis of mitochondrial complex assembly

Project description:Pulse (P) - Chase (C) SILAC analysis of mitochondrial complex assembly Bogenhagen et al (2018) Cell Reports Pulse-chase times in hours (P#C#)

2020-01-03 | MSV000084750 | MassIVE

Pulse-chase SILAC of Mycobacterium tuberculosis infected THP-1 macrophages

Project description:Pulse chase SILAC was used to identify protein turnover within human macrophages infected with mycobacterium tuberculosis CDC1551, a ppe38-71 mutant strain, a complemented strain and an uninfected control.

2021-06-15 | PXD021166 | Pride

HEK293T cell degradome (pulse chase) analysis upon 12h Torin1 treatment. Related to Supplementary Table 3 from An et al (2020)

Project description:HEK293T Azidohomoalanine (AHA) Pulse Chase (degradome) experiment, were cells from various genetic background (WT, ATG7-/-, RB1CC1-/-) are treated or not with Torin1 for 12h. Samples are Biotin-click, and streptavidin enriched.

2020-04-30 | PXD017857 | Pride

HEK293T cell degradome (pulse chase) time course (5h, 10, 15h) analysis upon Torin1 treatment. Related to Supplementary Table 3 from An et al (2020)

Project description:HEK293T Azidohomoalanine (AHA) Pulse Chase (degradome) time course experiment, were cells from various genetic background (WT, ATG7-/-, RB1CC1-/-) are treated or not with Torin1 for 5h, 10h or 15h. Samples are Biotin-click, and streptavidin enriched, digested and labeled with TMTpro 16plex.

2020-04-30 | PXD018158 | Pride

In vivo pulse-chase in C. elegans reveals intestinal histone turnover changes upon starvation

Project description:In vivo pulse-chase in C. elegans reveals intestinal histone turnover changes upon starvation

| PRJNA1224425 | ENA